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국산 제 2세대 혈소판 보존백을 이용한 5일 보존 혈소판농축액의 평가

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Author(s)
최현식장순희이난영서장수이원길김재식전동석김종규송달효
Keimyung Author(s)
Jeon, Dong Seok
Department
Dept. of Laboratory Medicine (진단검사의학)
Journal Title
대한임상병리학회지
Issued Date
1997
Volume
17
Issue
1
Keyword
PVCTEHTMIn vitro platelet function5-day stored platelet container
Abstract
Background :The most important things for the prolonged storage of platelet concentrates are whether it can be supplied with adequate O2 and maintained at optimal pH condition, which is dependent on materials of platelet storage containers and various plastcizers. Polyvinylchloride (PVC) containers plasticized with tri-(2-ethylhexyl) trimellitate (TEHTM) and polyolefin containers which can be stored for 5to 7 days are known as second-generation platelet storage containers, The authors evaluated a new domestic platelet storage container made of PVC plasticized with TEHTM. Method :20 units of platelet concentrates were stored in PVC containers plasticized with TEHTM (BOIN MEDICA Corporation, Korea) which were mixing on flat-bed platelet agitator for 7 days at room temperature. In vitro, platelet count, MPV,PDW, PH, PO2, pC02, HCO3-, Na+, K+, Cl-, LDH, lactate, hypotonic shock response(HSR), extended shape change (ESC), platelet morphology change (PMC) and platelet aggregation test were evaluated at day 0, 2, 5, and day 7. Also, all platelet concentrates ware cultured at day 0. Result :There were no growth in all bacterial cultures. In contrast to day 0, mean platelet count, mean platelet volume (MPV) , platelet distribution width(PDW), p02, pC02, HCO3- and Na+, K+, Cl- did not show significant unfavorable changes at day 5 and pH was well maintained at day 5 (7.33) and at day 7 (7.14). LDH and lactate increased proportionally to storage time. HSR and ESC were 60%, 57% at day 5 and 38%, 40% at day 7 and it was similar to that of platelet aggeregation test (at day 7: collagen 58%, ADP+collagen+epinephrine 54%) in contrast to day 0. Platelet morphology change (PMC) was well maintained as non-discoid platelet which was counted as 38% at day 5 and 54% at day 7. Conclusion :In vitro platelet function of platelet storage container, pH was maintained above 7.0 for 7 days and HSR, ESC, PMC and platelet aggregation function were well maintained for 5 days. As a result of the study, the authors concluded that the use of 5-day stored CPDA-1 platelet container plasticized with TEHTM in Korea is recommendable for alleviating the difficulties in supply of platelet concentrates.
Alternative Title
Evaluation of Platelet Concentrates Stored for Five Days in Domestic Second Generation Platelet Storage Containers
Keimyung Author(s)(Kor)
전동석
Publisher
School of Medicine
Citation
최현식 et al. (1997). 국산 제 2세대 혈소판 보존백을 이용한 5일 보존 혈소판농축액의 평가. 대한임상병리학회지, 17(1), 173–182.
Type
Article
ISSN
1015-6445
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/39429
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학)
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