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HaCaT 각질형성세포에서 자외선 B에 대한 세포자멸사 및 자가포식현상 유도

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Author(s)
윤상돈백원기김상표이규석조재위
Keimyung Author(s)
Lee, Kyu SukCho, Jae WeBaek, Won KiKim, Sang Pyo
Department
Dept. of Dermatology (피부과학)
Dept. of Microbiology (미생물학)
Dept. of Pathology (병리학)
Journal Title
대한피부과학회지
Issued Date
2013
Volume
51
Issue
8
Keyword
ApoptosisAutophagyHaCaT cellUVB
Abstract
Background: UVB irradiation induces apoptosis or/and autophagy through several molecular pathways in keratinocytes. However, the precise molecular mechanism of UVB-induced autophagy is largely unknown in keratinocytes.
Objective: The purpose of this study was to investigate the molecular mechanisms of UVB-induced apoptosis and autophagy in HaCaT cell lines. Methods: Cells were irradiated by UVB (Westinghouse FS-40 sunlamps) with various doses (0, 30, 60, 120, 240 mJ/cm2). The expression levels of caspase-3, Bax, Bcl2, Bcl-XL and LC3 were confirmed by Western blot analysis in UVB-irradiated HaCaT cell lines. Apoptotic cells were analyzed by PI staining, and autophagy cells were analyzed by immunofluorescent staining. Results: The expression of Bcl-XL decreased from UVB 60 mJ/cm2 and Bcl2 decreased from UVB 240 mJ/cm2. The expression of caspase-3 was increased from UVB 120 mJ/cm2
. These data showed that UVB-induced apoptosis is mediated by up-regulation of caspase-3 and down-regulation of Bcl2 and Bcl-XL. Furthermore, the expression of LC3 increased from UVB 120 mJ/cm2. In addition, autophagy formation was observed in few fractions of apoptotic HaCaT cells in immunofluorescent staining; most apoptotic cells did not show autophagy formation. Moreover, autophagy formation inhibitor treatment induced a slight increment of apoptotic cell population under UVB irradiation. Conclusion: UVB irradiation induces not only apoptotic cell death but also autophagy formations; these events may create a defense mechanism for the prevention of apoptosis in UVB-treated HaCaT cells.
Alternative Title
Induction of Apoptosis and Autophagy in UVB-Treated HaCaT Cells
Keimyung Author(s)(Kor)
이규석
조재위
백원기
김상표
Publisher
School of Medicine
Citation
윤상돈 et al. (2013). HaCaT 각질형성세포에서 자외선 B에 대한 세포자멸사 및 자가포식현상 유도. 대한피부과학회지, 51(8), 600–607.
Type
Article
ISSN
0494-4739
Source
http://kiss.kstudy.com/thesis/thesis-view.asp?key=3156241
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/39778
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Dermatology (피부과학)
1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
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