HaCaT 각질형성세포에서 자외선 B에 의하여 유도되는 염증성 사이토카인에 대한 Curcumin의 항염증 효과

Abstract

Background: UVB irradiation induces acute inflammation through expressions of IL-6, IL-8, and TNF-α, in keratinocytes. Curcumin, extracted from the rhizomes of Curcuma longa, has been described as having anti-inflammatory properties; however, it still unknown as to whether curcumin inhibits the UVB-induced expressions of IL-6, IL-8, and TNF-α in HaCaT cell lines. Objective: In this study we investigated the effects of curcumin on the expression of IL-6, IL-8, and TNF-α in HaCaT cells, as well as the photoprotective effect of curcumin ointment in UVB-irradiated hair less mice. Methods: HaCaT cells were irradiated with various doses of UVB (0, 100, 200, 300 mJ/cm2). Total RNA was extracted from the cells and gene expression was confirmed by RT-PCR, EMSA, and Western blot analysis. In addition we examined whether the ointment containing curcumin prevents the photo damaging events in hairless mice. Results: UVB-irradiated HaCaT cells clearly showed an increased expression of IL-6, IL-8, and TNF-α in a UVB dose-dependent manner (0, 100, 200, 300 mJ/cm2). Interestingly, pretreatment of curcumin (40 uM) dramatically reduced the expressions of UVB-induced IL-6, IL-8, and TNF-α. In addition, activation of NF-κB and MAPKs (p38, JNK, ERK) by UVB were partially attenuated by pretreatment of curcumin in HaCaT cells. Furthermore, the application of ointment containing curcumin partially protected the erythema and skin atrophy in UVB-irradiated hairless nude mice. Conclusion: Collectively, these results indicate that curcumin may be applied as a promising anti-inflammatory natural agent through modulation of inflammatory cytokines such as IL-6, IL-8, and TNF-α.