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혈소판 풍부혈장을 처치한 섬유아세포에서 제 1형 교원질, MMP-1 및 MMP-2 발현 증가

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Author(s)
한길김상표조재위
Keimyung Author(s)
Cho, Jae WeKim, Sang Pyo
Department
Dept. of Dermatology (피부과학)
Dept. of Pathology (병리학)
Journal Title
대한피부과학회지
Issued Date
2015
Volume
53
Issue
4
Keyword
Platelet-rich plasmaSubcisionDermal remodeling
Abstract
Background: Platelet-rich plasma (PRP) treatment is a promising tool for dermal tissue regeneration. PRP combined with subcision can synergistically induce dermal tissue regeneration.
Objective: The purpose of this study was to investigate the effects of PRP on the proliferation and migration of skin fibroblasts, as well as on the type I collagen, matrix metalloproteinase (MMP)-1, and MMP-2 expression in
these skin cells. The effect of PRP with subcision on the expression of TGF-β1 was also investigated in an animal model. Methods: Human skin fibroblasts were treated with various concentrations of PRP. The proliferation and migration
rate of the cells were evaluated by the trypan blue exclusion method and scratch assay, respectively. The expression levels of type I collagen, MMP-1, and MMP-2 were analyzed by western blot or RT-PCR. In addition, the activity
levels of MMP-1 and MMP-2 were studied by zymography. Finally, we treated the animal back with PRP, subcision, or PRP with subcision. The specimens were evaluated by H&E, Masson-trichrome, and TGF-β1
immunohistochemical staining. Results: Data from this study showed that PRP more effectively promoted the migration and proliferation of cells in a dose-dependent manner. The expression levels of type I collagen, MMP-1, and MMP-2 were increased in PRP-treated fibroblasts at the protein and mRNA levels. The in vivo study revealed that the expression of TGF-β1 was prominently increased by co-treatment with PRP and subcision rather than by treatment with either PRP or subcision alone. Conclusion: PRP treatment promoted fibroblast migration and proliferation, and increased the expression of type I collagen, MMP-1, MMP-2, and TGF-β1. Therefore, PRP co-application with subcision is an effective method for dermal remodeling and can be a good treatment option for depressed acne scars.
Alternative Title
Increased Expression of Type Ⅰ Collagen, MMP-1, and MMP-2
in Platelet-rich Plasma-treated Human Skin Fibroblasts
Keimyung Author(s)(Kor)
조재위
김상표
Publisher
School of Medicine
Citation
한길 et al. (2015). 혈소판 풍부혈장을 처치한 섬유아세포에서 제 1형 교원질, MMP-1 및 MMP-2 발현 증가. 대한피부과학회지, 53(4), 290–297.
Type
Article
ISSN
0494-4739
Source
http://kiss.kstudy.com/thesis/thesis-view.asp?key=3323073
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/39781
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Dermatology (피부과학)
1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
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