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섬유모세포에서 G1 세포주기 정지에 따른 제1형 교원질 유전자 발현 감소

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Author(s)
김성애, 김병천 ,김창욱, 류영욱, 박의수, 송준영, 오병호, 이규석, 조재위
Keimyung Author(s)
Lee, Kyu Suk
Department
Dept. of Dermatology (피부과학)
Journal Title
대한피부과학회지
Issued Date
2007
Volume
45
Issue
4
Keyword
FibroblastG1 phase arrestType I collagen
Abstract
Background: Type I collagen is a major component of extracellular cellular matrix in tissue. It is synthesized by human skin fibroblasts. However, synthesis of type I collagen is markedly-decreased in senescent fibroblasts. In the
cell cycle, the hallmark of senescent fibroblasts is a permanent G1 phase arrest. However it is largely unknown whether the expression of type I collagen protein is decreased by the G1 phase arrest in human skin fibroblasts after
UVB irradiation, serum starvation, or mimosine, an inducer of the G1 phase arrest treatment. Objective: The purpose of this study was to investigate the expression of type I collagen protein in G1 phasearrested
human skin fibroblasts after UVB irradiation, serum starvation, and mimosine treatment. Methods: To induce G1 phase arrest in the cell cycle, human skin fibroblasts were irradiated by UVB (100, 200, 300 mJ/cm2), subjected to serum starvation for 5 days, or mimosine treatment (50, 100, 200 uM). The expressions of type I collagen protein were analyzed by Western blot analysis. Results: The G1 phase of cell populations were increased by a dose or time-dependent manner with UVB irradiation, serum starvation, and mimosine-treated human skin fibroblasts. The expression of type I collagen protein was markedly-decreased by UVB, serum starvation, and mimosine treatment.
Conclusion: The expression of type I collagen protein in human skin fibroblasts is decreased by UVB irradiation, serum starvation, and mimosine treatment through induction of G1 phase cell cycle arrest.
Alternative Title
Down-regulation of Type Ⅰ Collagen Gene Expression in Human Skin Fibroblasts by G1 Cell Cycle Arrest
Keimyung Author(s)(Kor)
이규석
Publisher
School of Medicine
Citation
김성애, 김병천 ,김창욱, 류영욱, 박의수, 송준영, 오병호, 이규석, 조재위. (2007). 섬유모세포에서 G1 세포주기 정지에 따른 제1형 교원질 유전자 발현 감소. 대한피부과학회지, 45(4), 362–368.
Type
Article
ISSN
0494-4739
Source
http://kiss.kstudy.com/thesis/thesis-view.asp?key=2607371
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/39834
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Dermatology (피부과학)
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