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전신성 경피증 피부섬유아세포에서 Ⅶ형 교원질 mRNA의 검출

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Author(s)
이규석신문석권호준송준영
Keimyung Author(s)
Lee, Kyu SukSong, Joon Young
Department
Dept. of Dermatology (피부과학)
Journal Title
대한피부과학회지
Issued Date
1996
Volume
34
Issue
4
Keyword
In situ hybridization RT-PCRSclerodermaType Ⅶ collagenAntibody
Abstract
Background: Type VII collagen is a relatively low abundance extracellular matrix protein among the collagenous molecules. Among the minor collagens. type VII collagen has been demon strated by a immunolocalization studies to be component of anchoring fibrils and structures extending perpendicularly from the lamina densa to the upperpapillary dermis. Objective : The purpose of his study is to determine the expression of the type VII collagengene in a group of scleroderma patients as compared to normal skin. Methods : We have examined the levels of type VII collagen mRNA using quantitative reverse transcription PCR and in sit gybridization in scleroderma skin fibroblasts. Immunofluorescent staining with anti-type VII collan antibody was performed in vitro and in vivo to evaluate the expression of type VII collagen at protein level. Results : 1. the ratio of type VII collagen/GAPDH RT-PCR product were 63.3+15.3 in scleroderma and 21.7+7.6 in normal fibroblasts by RT-PCR. 2. The expression of type VII collagen mRNA was considerably lower than type I in scleroderma. A few positive signals by in situ hybridization with type VII collagen cDNA were shown in the dermis. 3. The staining was markedly enhanced in scleroderma fibroblasts and tissues compaired with normal subjects in imunofluorescent staining with anti-Type VII collagn antibody. Conclusion : RT-PCR and immunofluorescent staining with antibodies to type VII collagen shows enhanced gene expression in scleroderma skin fibroblasts. These data suggest that type VII collagen may be the main soruce of the sclerotic change of skin in scleroderma.
Alternative Title
Detection of Type Ⅶ Collagen mRNA in Scleroderma Fibroblasts
Keimyung Author(s)(Kor)
이규석
송준영
Publisher
School of Medicine
Citation
이규석 et al. (1996). 전신성 경피증 피부섬유아세포에서 Ⅶ형 교원질 mRNA의 검출. 대한피부과학회지, 34(4), 591–599.
Type
Article
ISSN
0494-4739
Source
http://kiss.kstudy.com/thesis/thesis-view.asp?key=1871239
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/39865
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Dermatology (피부과학)
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