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편평상피암에서의 c-myc, erb B 및 EGF-Receptor 유전자의 세포-분자생물학적 특성

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Author(s)
이규석류영욱최윤애송준영최인장장성익백원기서민호
Keimyung Author(s)
Lee, Kyu SukRyoo, Young WookSong, Joon YoungChoi, In JangChang, Sung IkBaek, Won KiSuh, Min Ho
Department
Dept. of Dermatology (피부과학)
Dept. of Anatomy (해부학)
Dept. of Microbiology (미생물학)
Journal Title
대한피부과학회지
Issued Date
1994
Volume
32
Issue
2
Keyword
c-mycCytogeneticEGFRerb BSCC
Abstract
Background : Oncogenes and EGF-Receptor(EGFR) may be involved n different stages of the multistep carcinogenesis process. A specific pattern of karyotypic abnormalities in solid tumors can be detected by cytogenetic methods. Onjective : This study is intnded to observe the cytomolecular kiologic chracterization of c-myc, erb B and EGFR genes in squasnous cell carcinoma(SCC) of the skin and cervix. Methods : We have eytogenet,ically examined the short-term culturs from SCC. The rearrangement, amplification or expressi.on of erb B, c-myc, and EGFR genes were studied by Southern blot, analysis of genomic DNA and by slot blot analysis of tota! RNA extracted from biopsies of normal skin and SCC tissues. EGFR expression was examined immunohistochemially using monoclonal antibodies and the localizat,ion of the c-myc oncogene mRNA by in situ hybridization. Results : A remarkably structural aberration was del 6(q21-qter) counted 20 metaphases among 28 metaphases ana1yzed. In nunierical aberration, all chromosomes were lost or gained randomly. Amenploid including triploid and tetraploid were observed in 8 metaphases, 6 tumor cells contained marker chromosome. In Southern blot analysis, rearrangement and amglificaton of EGFR in primary squamous cell carcinoma of cervix uteri and skin respectively. In slot blot analysis, the levels of c-myc, erb B and EGFR mRNA increaaed respectively 3.5, 2.5 and 2.8 times in SCC when compared to normal tissues. In immunoperoxidase stain, EGFR was present, in SCC where keratinocytes with strong cyto-plasmic staining but no membr, line labelling, where as in normal skin the were primarily present in t,he membrane and cytoplasm of basal cells. In situ hybridization with c-myc cDNAs allowed detection of grains representative of biotin labelled cDNA-mRNA hybrids in the frozden section of SCC tissues. Conclusion : These results suggest that specific patterns of karyotypir abnormalites, rearrangement, or amplification of EGFR gene, and overexpression of oncogenes and EGFR gene may be associated with the carcinogenesis of SCC.
Alternative Title
Cyto-molecular Biologic Characterization of c-myc, erb B and EGF-Receptor in Squamous Cell Carcinoma
Keimyung Author(s)(Kor)
이규석
류영욱
송준영
최인장
장성익
백원기
서민호
Publisher
School of Medicine
Citation
이규석 et al. (1994). 편평상피암에서의 c-myc, erb B 및 EGF-Receptor 유전자의 세포-분자생물학적 특성. 대한피부과학회지, 32(2), 223–233.
Type
Article
ISSN
0494-4739
Source
http://kiss.kstudy.com/thesis/thesis-view.asp?key=1870838
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/39882
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Anatomy (해부학)
1. School of Medicine (의과대학) > Dept. of Dermatology (피부과학)
1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
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