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흰쥐 피부창상에서 AP-1 decoy에 의한 교원질 생성효과: 반흔의 유전자 치료 가능성

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Author(s)
박수정이규석
Keimyung Author(s)
Lee, Kyu Suk
Department
Dept. of Dermatology (피부과학)
Journal Title
대한피부과학회지
Issued Date
2005
Volume
43
Issue
4
Abstract
BACKGROUND: The wound healing process is composed of inflammation, cellular growth, migration, angiogenesis and an extracellular matrix composition. In this process, fibroblasts proliferate but leave scarring due to their excessive growth. The process is controlled by platelet derived growth factor (PDGF) and basic fibroblast growth factor (bFGF), while PDGF and transforming growth factor beta(TGF-beta) play a major role in controlling extracellular matrix composition. Numerous modalities have been tried to treat this abnormal response, but the results were unsatisfactory. TGF-beta is activated by AP-1 (Activator protein). Theregone, AP-1 decoy oligodeoxynucleotides(ODN) had recently been used for regulation of TGF-beta transcription as a gene treatment. OBJECTIVE: The purpose of this study was to examine the effect of blocking TGF-beta transcription by an AP-1 decoy ODN on collagen synthesis in wound healing on rat skin. METHODS: In this study, the effect of AP-1 decoy ODN on collagen synthesis was examined by Hematoxylin and eosin (H&E) staining, Masson's trichrome staining, and immunohistochemical staining for TGF-beta on damaged rat skin. RESULTS: In the H&E stain and Masson's trichrome stain of the damaged rat skin, the number of collagen fibers of AP-1 decoy ODN treated group had decreased in compared to the control group, especially on the 15th day after incision. With immunohistochemical stain, the expression of TGF-beta in fibroblasts, inflammatory cells and the endothelium of vessel walls in the dermis had also decreased, compared to the control group. TGF-beta was expressed in the dermis from the 3rd day, and predominantly in the fibroblasts on the 15th day after incision.
Alternative Title
The Effect of an AP-1 decoy on Collagen Synthesis in Wound Healing on Rat Skin: The use of Gene Therapy to Minimise Scarring
Keimyung Author(s)(Kor)
이규석
Publisher
School of Medicine
Citation
박수정 and 이규석. (2005). 흰쥐 피부창상에서 AP-1 decoy에 의한 교원질 생성효과: 반흔의 유전자 치료 가능성. 대한피부과학회지, 43(4), 435–441.
Type
Article
ISSN
0494-4739
Source
http://kiss.kstudy.com/thesis/thesis-view.asp?key=2437216
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/39894
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Dermatology (피부과학)
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