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배양한 피부섬유아세포에서 bFGF와 TGF-β가 탄력소와 제1형 교원질 유전자의 전사조절에 미치는 영향

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Author(s)
류영욱
Keimyung Author(s)
Ryoo, Young Wook
Department
Dept. of Dermatology (피부과학)
Journal Title
대한피부연구학회지
Issued Date
1999
Volume
6
Issue
4
Keyword
Basic fibroblast growth factor(bFGF)ElastinCollagen
Abstract
Basic fibroblast growth factor(bFGF), a member of the fibroblast growth factor family, potentially induces increased vascular smooth muscle cell proliferation and decreased synthesis of collagen. A large number of extracellular matrix proteins including collagens, proteoglycans, elastin, fibronectin and fibrillins are produced by skin dermal fibroblasts. Skin fibroblasts have been extensively utilized for the diagnosis of connective tissue disease as well as aging. Elastin is one of the major extracellular matrix components of the dermis and plays an important role in providing elasticity and resilience of the skin. For investigation of the effect of bFGF on extracellular matrix homeostasis in the skin, we evaluated the expression of elastin and type I collagen from cultured dermal fibroblast at the transcriptional level. Using Northern blot analysis, transient transfection and CAT assay, we found that bFGF treatment caused decreases of elastin and type I collagen gene expression with dose and time dependent pattern. The effects of TGF-β, known as a potent fibrogenic cytokine, were inhibited by bFGF. Taken together, these results indicated that recombinant human bFGF decreased the elastin and type I collagen gene expression at pretranscriptional level and it showed antagonistic effect to TGF-β.
Alternative Title
Transcriptional Regulations of Elastin and Type Ⅰ Collagen Gene by bFGF and TGF-β in Cultured Skin Fibroblasts
Keimyung Author(s)(Kor)
류영욱
Publisher
School of Medicine
Citation
류영욱. (1999). 배양한 피부섬유아세포에서 bFGF와 TGF-β가 탄력소와 제1형 교원질 유전자의 전사조절에 미치는 영향. 대한피부연구학회지, 6(4), 232–238.
Type
Article
ISSN
1225-8180
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/39900
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Dermatology (피부과학)
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