피부종양에서의 nm23유전자의 발현

Abstract

The nm23 gene is a potential suppressor of matastasis. It is reported that nm123 mRNA levels are highest in normal and tumor cells of low metastatic potential, providing the first evidence that metastasis, in an experimental system, is associated with the loss of activity of nonimmunologcally related gene. The purpose of the study is to determine the expression of the nm23 gene in three squamous cell carcinoma, four basal cell carcinoma and two malignant melanoma cases. In this study, we have examined the levels of nm23 mRNA using reverse transcriptase polymerase chain reaction(RT-PCR) and expression of nm23 oncoprotein using immunoperoxidase stain with monoclonal antibody in three squamous cell carcinoma, four basal cell carcinoma and two malignant melanoma cases. The results were as follows; 1.In RT-PCR and digoxigenin hybridization, the expressions of nm23 gene were increased in three squamous cell carcinoma, four basal cell carcinoma cases, but expressions were reduced in two malignant melanoma cases. 2.In immunoperoxdase staining by monoclonal anti-nm23 antibody, expression of nm23 oncoprotein was in general lower in squamous cell carcinoma and basal cell carcinoma except malignant melanoma than in normal epithelium. 3. In immunoperoxdase staining by monoclonal anti-nm23 antibody, nm23 expression of squamous cell carcinoma, basal cell carcinoma, malignant melanoma was predominantly detected at various degrees in cytoplasms of tumor cells. In conclusion, our data suggest that the correlation between the data of immunohistochamical analysis for nm23 gene and the nm23 mRNA levels is weak, so we can not consider a biologcal marker of potential suppressor in cutaneous carcinogenesis.