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Change in Expression of Keratin and Proto-oncogene Induced by Beta-propiolactone in HaCaT Cell

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Author(s)
Yin LiuEon-Gi SungIn-Hwan SongDongyi DuDae-Kwang KimJeong-Hyun ParkHoon-Ki SungYungchang LeeJoo-Young Kim
Keimyung Author(s)
Kim, Dae Kwang
Department
Dept. of Medical Genetics (의학유전학)
Journal Title
대한해부학회지
Issued Date
2001
Volume
34
Issue
4
Keyword
Keratins (K8, K10, K13)Proto-oncogenes(c-fos, c-jun, c-myc)Beta-propiolactoneHaCaT
Abstract
To investigate the relationship between the morphologic changes and the expression of keratin and proto-oncogene induced by Beta-propiolactone (BPL), we assessed on the expression of keratins (K8, K10, K13) and proto-oncogenes (c-fos, c-jun, c-myc) in human HaCaT cell line. The cells were treated with 0, 0.1, 1 microgram/ml BPL for 2 or 6 hours. Morphologic studies revealed that BPL changed the cells into fibrocyte-shaped, caused highly lobulated nuclei and reduced desmosomes in their number. Findings of immunofluorescence and Northern blotting indicated that BPL consistently decrease expression of K10 representing a normal differentiation marker of keratinocytes, while increasing expression of K8 and K13 associated with a pathologic differentiation. This reagent also up-regulated expression of c-fos and c-jun, and down-regulated expression of c-myc. Together with staining for each keratin or proto-oncogene and DNA content in flow cytometry, BPL increased K8 expression dramatically at S-G2-M phase. The induction of c-Fos at S-G2-M phase appeared within 2 hours, and c-Jun gradually occurred. However, c-Myc was inhibited regardless of phases of cell cycle. In conclusion, these changes caused by BPL demonstrate a close relationship between the morphologic evidence and the altered expression of each keratin and proto-oncogene.
Alternative Title
Beta-propiolactone에 의한 사람각질화세포(HaCaT)의 Keratin과 Proto-oncogene 발현의 변화
Keimyung Author(s)(Kor)
김대광
Publisher
School of Medicine
Citation
Yin Liu et al. (2001). Change in Expression of Keratin and Proto-oncogene Induced by Beta-propiolactone in HaCaT Cell. 대한해부학회지, 34(4), 389–404.
Type
Article
ISSN
1225-1305
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/39908
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Medical Genetics (의학유전학)
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