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Thallium-201을 이용한 세포막 Na+-K+ ATPase 활성도 측정: Rubidium-86 측정법과의 비교

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Author(s)
이재태이인규손상균이규보
Keimyung Author(s)
Lee, In Kyu
Department
Dept. of Internal Medicine (내과학)
Journal Title
대한핵의학회지
Issued Date
1998
Volume
32
Issue
2
Keyword
Na+-K+ ATPase activityThallium-201Rubidium-86
Abstract
Purpose: Na+-K+ ATPase Activity has beem estimated by the degree of inhibition of cation transport by cardiac glycosides (ouabain) using Rb-86 as a substrate. The biological characterist- Isc of T1-201 is known to be simiIar to those of potassium as a transport substrate in the presence of glucose, insulin or phobol myristate acetate (PMA). The purpose of this study was to measure ouabain sensitive Na+-K+ ATPase activity using T1-201 and compare with that using Rb-86. Materials and Methods: Smooth muscle cells isolated from rat aorta or human placental umbilical artery were cultured, and used to measure cellular Na+-K+ ATPase activity. Na+-K+ ATPase activity was measured as a percentage decrease in cellular uptake of T1-201 or Rb-86 by ouabain under the presence of glucose, insulin or PMA in media. Results: Na+-K+ ATPase ase activity measured with T1-201, as a transport substrate, was not different from those measured with Rb-86 in rat or human smooth muscle cell preparation. Incubation with high concentration glucose resulted in about 30% decrease in enzyme activity. In contrast, insulin or PMA resulted in 50-70% or 28% increases from baseline activity, respectively. Conclusion: These results suggests that T1-201 could replace Rb-86 in measurement of ouabain sensititive Na+-K+ ATPase activity in vitro. High level of glucose concentration decreased cellular Na+-K+ ATPase activity, but insulin or PMA increased it.
Alternative Title
Quantitative Measurement of Membrane Na+-K+ ATPase Activity using Thallium-201: Comparison with Rubidium-86
Keimyung Author(s)(Kor)
이인규
Publisher
School of Medicine
Citation
이재태 et al. (1998). Thallium-201을 이용한 세포막 Na+-K+ ATPase 활성도 측정: Rubidium-86 측정법과의 비교. 대한핵의학회지, 32(2), 121–128.
Type
Article
ISSN
1225-6714
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/39941
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
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