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제대혈 조혈모세포 은행 설립을 위한 제대혈 분리방법의 표준화에 관한 연구

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Author(s)
이영호김흥식이건수국훈황태주박경덕조빈김학기성기웅신희영안효섭최용묵
Keimyung Author(s)
Kim, Heung Sik
Department
Dept. of Pediatrics (소아청소년학)
Journal Title
대한혈액학회지
Issued Date
1999
Volume
34
Issue
2
Keyword
Cord blood bankStandardization3% gelatinFicoll-Hypaque
Abstract
BACKGROUND: To standardize the separation method of cord blood and establish the Korean Cord Blood Bank, we analysed the data regarding cord blood separation from 8 different hospitals which involved in cord blood banking project.
METHODS: We analysed 306 samples which collected with heparinized syringes and separated within 24 hours by Ficoll-Hypaque density gradient separation method and RBC depletion method with 3% gelatin. We performed the mononuclear cell(MNC) court, viability, CD34+ cell count and CFU-GM count following separation of cord blood. We assessed the yield of hematopoietic stemcells by different separation methods in 9 hospitals and the variability among hospitals which performed cord blood separation by same method.
RESULTS: 1) In 204 samples of cord blood separated with Ficoll-Hypaque, there were significant differences in MNC count, viability, CD34+ cell count and CFU-GM count among all engaged hospitals, but not in each individual hospital. 2) In 102 samples of cord blood separated with 3% gelatin, there were significant differences in viability, CFU-GM count and CD34+ cell count among all engaged hospitals, but not in MNC count. 3) The yield of MNCs, CD34+ cells and CFU-GM was higher in the cord blood separated with 3% gelatin than Ficoll-Hypaque.
CONCLUSION: We suggest that RBC depletion method with 3% gelatin would be better than Ficoll-Hypaque method in order to increase the yield of hematopoietic stem cells and standardize the method of cord blood separation.
Alternative Title
Standardization of Cord Blood Separation Method for Establishment of Korean Cord Blood Bank
Keimyung Author(s)(Kor)
김흥식
Publisher
School of Medicine
Citation
이영호 et al. (1999). 제대혈 조혈모세포 은행 설립을 위한 제대혈 분리방법의 표준화에 관한 연구. 대한혈액학회지, 34(2), 235–240.
Type
Article
ISSN
1225-0546
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/39985
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Pediatrics (소아청소년학)
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