Candida의 항진균제 감수성과 DNA특성

Abstract

Twenty-six strains of Candida albicans isolated from various clinical specimens were tested for antifungal susceptibility to 5 drugs, amphotericin B(AMB), nystatin (NST), clotrimazole (CTZ), ketoconazole (KCZ) and flucytosine (5-FC). The range of minimum inhibitory concentration (MIC) of the drugs were as follows : A MB, 0.25¬1μg/m\ ； NST, 8-32μg/ml;CTZ, <0.125-4μg/ml;KCZ, <0.125-〉128μg/ml ； 5-FC, 8->1024μg/ml. 90 % MICs of the drugs to Candida albicans were 0.47μg/ml for AMB, 1.9/μg/ml for CTZ, 14.9μg/ml for NST,〉128μg/ml for KCZ and >1024μg/ml for 5-FC. Restriction enzyme analysis (REA) using restriction endonuclease BamHI was performed to char¬acterize the genomic DNA of C. albicans in molecular aspect. The resulting gel patterns of REA revealed five intensely stained bands with ethidium bromide (8.7Kb, 7.6Kb, 6.6Kb, 6.0K, 3,6Kb). These intense band patterns produced three strain types (group A, B and C). Group A had 8.7Kb, 6.6Kb, 3.6Kb bands. Group B had 8.7 Kb, 6.0Kb, 3.6 Kb bands and group C had 8.7 Kb, 7.6 Kb, 3.6 Kb bands. Among the total 26 strains, 21 strains be¬longed to group A. Group B had 3 strains and group C consisted of 2 strains. In order to elucidate the ras oncogene expression in C. albicans, southern hybridization of BamHI digested C. albicans genomic DNA with <z-32P-labeled v-Ha-ras probe prepared by nick translation was performed. The v-Ha-ras probe was hybridized to C. albicans genomic DNA at 4.9 Kb. Key Words I C. albicans，Chromosomal DNA, Restriction enzyme analysis (REA), Ras gene.