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HL60 세포주의 분화유도에 따른 Apoptosis관련 유전자의 발현

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Author(s)
백원기Won Ki Baek
Keimyung Author(s)
Baek, Won Ki
Department
Dept. of Microbiology (미생물학)
Journal Title
대한미생물학회지
Issued Date
1999
Volume
34
Issue
1
Abstract
In hematopoietic tissues, apoptosis is coupled to terminal differentiation of myeloid progenitors. The mechanism of apoptosis associated with differentiation is poorly understood. HL60, promyelocytic leukemia cell line, was used to study expression of apoptosis related genes (bcl-2, bcl-xivs, c-iap 2,and procaspase 3) during differentiation induced by 12-O-tetradecanoylphorbol- 13-acetate (TPA). HL60 cells were differentiated to monocyte/macrophage like cells about 1 day after TPA treatment, and differentiated cells were gradually induced apoptosis from 4 days after TP A treatment. The bcl-2 mRNA and protein product were rapidly and continuously downregulated but expression of bcl-XL and c-iap 2 genes were increased in early stage of differentiation and then decreased. Expression of bcl-xs, the alternative splicing form of bcl-xL gene, was slowly and continuously increased. These data suggest that increased expression of bcl-xL and c-iap 2 genes1 may provide a mechanism through which apoptosis is blocked in early stage of HL60 cell differentiation.
ᄎ韓微生物學會誌 第34卷 第1號 1999 J. Korean Soc. Microbiol,, Vol. 34,No. 1, 1999
Key Words: Differentiation, Apoptosis, HL60, bcl-2, bcl-x, c-iap 2,Procaspase 3
Alternative Title
Expression of Apoptosis Related Genes During HL60 Cell Differentiation
Keimyung Author(s)(Kor)
백원기
Publisher
School of Medicine
Citation
백원기 and Won Ki Baek. (1999). HL60 세포주의 분화유도에 따른 Apoptosis관련 유전자의 발현. 대한미생물학회지, 34(1), 85–92.
Type
Article
ISSN
0253-3162
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/40151
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
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