인체대장암의 암유전자와 항암유전자의 분자유전적 특성 및 발현

Abstract

To define the role of cellular oncogenes and tumor suppressor genes in human colorectal canc¬ers, genetic alterations of ras, myc, fos oncogenes and p53 antioncogene in 30 freshly isolated primary colorectal cancer tissues were studied by using various molecular methods. In Southern blot hybridization, modest amplifications of c-myc gene(40%) were found in cancer tissues but no amplification and rearrangement of c-Ha-ras, c-fos and p53 genes were observed. In RNA slot blot analysis, 47% of cases showed significantly elevated expression of c-myc gene and 12% of cases showed modest elevated level of c-Ha-ras gene transcripts. These results suggested that amplification/overexpression of c-myc gene and elevated expression of c-Ha-ras gene were involved in tumor development of Korean colorectal cancers. Key Words: Colorectal cancer, ras, myc, fos, p53.