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한국인 대장-직장암에서 Ras 암유전자의 점돌연변이

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Affiliated Author(s)
백원기서성일서민호
Alternative Author(s)
Baek, Won KiSuh, Seong IlSuh, Min Ho
Journal Title
대한미생물학회지
ISSN
0253-3162
Issued Date
1995
Abstract
The point mutations of ras oncogene in human tumor have been well documented. These mu¬tations have been exclusively localized to codon 12,13 and 61 of three ras genes(K-, H-,N-ras). We have evaluated the point mutation of c-K-ras and c-Ha-ras codon 12 in Korean colorectal cancers using recently developed PCR(Polymerase Chain Reaction) - RFLP(Restriction Fragment Length Polymorphism) method.
The presence of mutations at codon 12 of c-Ha-ras and c-K-ras gene was examined by the en- dogeneous or primer mediated restriction fragment length polymorphism using polymerase chain reaction. Five of 30 colorectal cancers tested contained mutations at codon 12 of c-K-ras gene. On the other hand, no mutations were found at codon 12 of c-Ha-ras gene. The mutations were confirmed by PCR product cloning and nucleic acid sequencing. We think that PCR-RFLP method is accurate and time saving, and can be applied toward the rapid, non-radioactive screening of colorectal cancers.
Key Words: Ras, mutation, Colorectal cancer
Alternative Title
PCR-RFLP Analysis for Detecting Point Mutations at 12th Codon of Oncogene Ras in Korean Colotectal Cancers
Department
Dept. of Microbiology (미생물학)
Institute for Medical Science (의과학연구소)
Publisher
School of Medicine
Citation
백원기 et al. (1995). 한국인 대장-직장암에서 Ras 암유전자의 점돌연변이. 대한미생물학회지, 30(6), 718–725.
Type
Article
ISSN
0253-3162
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/40171
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
3. Research Institutues (연구소) > Institute for Medical Science (의과학연구소)
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