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Cyanate improves insulin sensitivity and hepatic steatosis in normal and high fat-fed mice: Anorexic and antioxidative effects

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Author(s)
Seong Sik KangKyo-Cheol MunJi Hae SeoMisun ChoeEunyoung Ha
Keimyung Author(s)
Mun, Kyo CheolHa, Eun YoungSeo, Ji HyeChoe, Mi SunKang, Seong Sik
Department
Kidney Institute (신장연구소)
Dept. of Biochemistry (생화학)
Dept. of Pathology (병리학)
Dept. of Internal Medicine (내과학)
Journal Title
Chemico-Biological Interactions
Issued Date
2018
Volume
279
Keyword
CyanateObesityOxidative stressReactive oxygen species
Abstract
Obesity is an important contributing factor to progression of chronic kidney disease. Cyanate, known as uremic toxin, is an electrophile produced spontaneously from urea or by myeloperoxidase-catalyzed oxidation of thiocyanate. Herein, we explored metabolic effects of cyanate in normal chow diet (NCD)- and high fat diet (HFD)-fed mice. Mice were treated with cyanate (1 mg/mL in drinking water) and fed NCD or HFD. Peritoneal glucose tolerance test (PGTT) and insulin tolerance test (ITT) were performed. Blood urea nitrogen (BUN) and creatinine concentrations were determined. Kidney and liver tissues were analyzed for reactive oxygen species (ROS) and lipid accumulations. Human albumin was carbamylated and evaluated for ROS scavenging activities. Contrary to our expectations, we found that cyanate treatment improved increased insulin sensitivity and alleviated hepatic steatosis in NCD- and HFD-fed mice. PGTT and ITT revealed faster and immediate glucose clearance in cyanate-treated NCD- and HFD-fed mice. Histological analysis of kidney and serum levels of BUN and creatinine showed no significant differences between cyanate-treated and control mice groups. Cyanate treatment reduced appetite and body weight in both NCD- and HFD-fed mice groups. Cyanate also decreased lipid peroxidation levels in the sera and the kidney, attenuated ROS levels in the kidney, which lead us to the findings that cAlb significantly reduced ROS levels compared to Alb in Caki-1 kidney and human umbilical vein endothelial cells. The results in this study may indicate that cyanate improves insulin sensitivity and hepatic steatosis possibly via exerting anorexic and antioxidative effects.
Keimyung Author(s)(Kor)
문교철
하은영
서지혜
최미선
강성식
Publisher
School of Medicine (의과대학)
Citation
Seong Sik Kang et al. (2018). Cyanate improves insulin sensitivity and hepatic steatosis in normal and high fat-fed mice: Anorexic and antioxidative effects. Chemico-Biological Interactions, 279, 121–128. doi: 10.1016/j.cbi.2017.10.026
Type
Article
ISSN
0009-2797
Source
https://linkinghub.elsevier.com/retrieve/pii/S0009-2797(17)30598-7
DOI
10.1016/j.cbi.2017.10.026
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/41167
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Biochemistry (생화학)
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
3. Research Institutues (연구소) > Kidney Institute (신장연구소)
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