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Development of a column-switching LC-MS/MS method of tramadol and its metabolites in hair and application to a pharmacogenetic study

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Author(s)
Hyerim YuMinje ChoiJung-Hee JangByoungduck ParkYoung Ho SeoChul-Ho JeongJung-Woo BaeSooyeun Lee
Keimyung Author(s)
Jang, Jung Hee
Department
Dept. of Pharmacology (약리학)
Journal Title
Archives of Pharmacal Research
Issued Date
2018
Volume
41
Issue
5
Keyword
TramadolLC-MS/MSCYP2D6Drug abuseGenetic polymorphismHair analysis
Abstract
Hair is a valuable specimen for monitoring long-term drug use. Tramadol is an effective opioid analgesic but is associated with risks such as drug dependence and unexpected toxicity arising from genetic differences in metabolism. However, few studies have been performed on the distribution of tramadol and its metabolites in hair. In the present study, a column-switching LC-MS/MS method was developed and fully validated for the simultaneous determination of tramadol and its phase I and II metabolites in hair. Furthermore, the distribution of tramadol and its metabolites in hair was investigated in a pharmacogenetic study. Tramadol and its metabolites were extracted from hair using methanol and injected onto LC-MS/MS. The validation results of selectivity, matrix effect, linearity, precision and accuracy were satisfactory. The (mean) concentrations of O-desmethyltramadol (ODMT) and N,O-didesmethyltramadol (NODMT) in the CYP2D6*10/*10 and CYP2D6*5/*5 groups were lower than those in the CYP2D6*wt/*wt group, while the (mean) concentrations of N-desmethyltramadol (NDMT) were higher. Moreover, the ratios of ODMT/tramadol, NDMT/tramadol and NODMT/NDMT were well correlated with the CYP2D6 genotypes. The developed method was successfully applied to the clinical study, which demonstrated that the concentrations of a drug and its metabolites in hair were dependent on the polymorphism of its metabolizing enzyme.
Keimyung Author(s)(Kor)
장정희
Publisher
School of Medicine (의과대학)
Citation
Hyerim Yu et al. (2018). Development of a column-switching LC-MS/MS method of tramadol and its metabolites in hair and application to a pharmacogenetic study. Archives of Pharmacal Research, 41(5), 554–563. doi: 10.1007/s12272-018-1013-7
Type
Article
ISSN
0253-6269
Source
https://link.springer.com/article/10.1007%2Fs12272-018-1013-7
DOI
10.1007/s12272-018-1013-7
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/41178
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Pharmacology (약리학)
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