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Improved application of the electrophoretic tissue clearing technology, CLARITY, to intact solid organs.

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Author(s)
Hyunsu LeeJae-Hyung ParkIncheol SeoSun-Hyun ParkShin Kim
Keimyung Author(s)
Lee, Hyun SuPark, Jae HyungKim, Shin
Department
Dept. of Anatomy (해부학)
Dept. of Immunology (면역학)
Dept. of Physiology (생리학)
Journal Title
BMC Developmental Biology
Issued Date
2014
Volume
14
Keyword
CLARITY, Brain, Nervous system, Electrophoretic tissue clearing, 3D Reconstruction, Purkinje layer
Abstract
BACKGROUND:

Mapping of tissue structure at the cellular, circuit, and organ-wide scale is important for understanding physiological and biological functions. A bio-electrochemical technique known as CLARITY used for three-dimensional anatomical and phenotypical mapping within transparent intact tissues has been recently developed. This method provided a major advance in understanding the structure-function relationships in circuits of the nervous system and organs by using whole-body clearing. Thus, in the present study, we aimed to improve the original CLARITY procedure and developed specific CLARITY protocols for various intact organs.

RESULTS:

We determined the optimal conditions for reducing bubble formation, discoloration, and depositing of black particles on the surface of tissue, which allowed production of clearer organ images. We also determined the appropriate replacement cycles of clearing solution for each type of organ, and convincingly demonstrated that 250-280 mA is the ideal range of electrical current for tissue clearing. We then acquired each type of cleared organs including brain, pancreas, liver, lung, kidney, and intestine. Additionally, we determined the images of axon fibers of hippocampal region, the Purkinje layer of cerebellum, and vessels and cellular nuclei of pancreas.

CONCLUSIONS:

CLARITY is an innovative biochemical technology for the structural and molecular analysis of various types of tissue. We developed improved CLARITY methods for clearing of the brain, pancreas, lung, intestine, liver, and kidney, and identified the appropriate experimental conditions for clearing of each specific tissue type. These optimized methods will be useful for the application of CLARITY to various types of organs.
Keimyung Author(s)(Kor)
이현수
박재형
김신
Publisher
School of Medicine (의과대학)
Citation
Hyunsu Lee et al. (2014). Improved application of the electrophoretic tissue clearing technology, CLARITY, to intact solid organs. BMC Developmental Biology, 14, 781–781. doi: 10.1186/s12861-014-0048-3
Type
Article
ISSN
1471-213X
DOI
10.1186/s12861-014-0048-3
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/41295
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Anatomy (해부학)
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
1. School of Medicine (의과대학) > Dept. of Physiology (생리학)
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