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Inflammation, Impaired Motility, and Permeability in a Guinea Pig Model of Postoperative Ileus

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Author(s)
Yoo Jin LeeZahid HussainCheal Wung HuhYoung Ju LeeHyojin Park
Keimyung Author(s)
Lee, Yoo Jin
Department
Dept. of Internal Medicine (내과학)
Journal Title
Journal of Neurogastroenterology and Motility
Issued Date
2018
Volume
24
Issue
1
Keyword
IleusInflammationPermeability
Abstract
Background/Aims:

Postoperative ileus (POI) is characterized by impaired propulsive function of the gastrointestinal tract after surgery. Although inflammation is considered to be an important pathogenesis of POI, significant data are lacking. We aim to correlate the recovery time of postoperative dysmotility with that of inflammation and mucosal permeability.

Methods:

An experimental POI model of guinea pig was used. Contractile activity of the circular muscle of the stomach, jejunum, ileum, and proximal colon was measured through a tissue bath study. Inflammatory cells were counted, and the expression of calprotectin and tryptase were analyzed. The expression of protease-activated receptor 2 (PAR-2), claudin-1, and claudin-2 were analyzed with immunofluorescence.

Results:

The small bowel and colon showed decreased contractile amplitude in the POI groups compared to control. In contrast to the colon, the contractile amplitude of the small bowel significantly recovered in the POI group at 6 hours after the operation compared to the control group. Inflammation was highly significant in the POI groups compared to the control and sham groups, especially in the colon. Immunofluorescence showed increased PAR-2 expression in the POI groups compared to sham. The decreased claudin-1 expression and increased claudin-2 expression may suggest increased mucosal permeability of the small bowel and colon in the POI groups.

Conclusions:

Increased inflammation and mucosal permeability may play an important role in the differential recovery stages in POI. These data may provide further insights into the pathophysiology and potential new therapeutic prospects of POI.
Keimyung Author(s)(Kor)
이유진
Publisher
School of Medicine (의과대학)
Citation
Yoo Jin Lee et al. (2018). Inflammation, Impaired Motility, and Permeability in a Guinea Pig Model of Postoperative Ileus. Journal of Neurogastroenterology and Motility, 24(1), 147–158. doi: 10.5056/jnm17012
Type
Article
ISSN
2093-0879
DOI
10.5056/jnm17012
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/41307
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
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