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Role of hair pigmentation in drug incorporation into hair

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Author(s)
Hyerim YuWon-Jun JangJung-Hee JangByoungduck ParkYoung Ho SeoChul-Ho JeongSooyeun Lee
Keimyung Author(s)
Jang, Jung Hee
Department
Dept. of Pharmacology (약리학)
Journal Title
Forensic Science International
Issued Date
2017
Volume
281
Keyword
Drug abuseHair analysisHair cellHair pigmentationMelaninTramadol
Abstract
Hair analysis has notably expanded its application as a bio-monitor for drug or toxicant exposure. Hair pigmentation is proposed as a major factor affecting drug incorporation into hair; however, the mechanisms underlying the incorporation of drugs into hair are still unclear. In the present study, the effect of hair pigmentation on drug incorporation into hair was examined using rats carrying hair with different melanin status and human cells (SK-Mel-28 cells, HaCaT cells and the co-cultured HaCaT cells with SK-Mel-28 cells) representing the main pigmentary unit in hair. Tramadol, a synthetic opioid analgesic, was selected as a model drug. The distribution of tramadol and its phase I (O-desmethyltramadol [ODMT], N-desmethyltramadol [NDMT] and N,O-didesmethyltramadol [NODMT]) and phase II metabolites (ODMT-glucuronide and NODMT-glucuronide) was investigated in non-pigmented and pigmented hair from Long-Evans rats. Moreover, the incorporation levels of ODMT and ODMT-glucuronide were compared in hair cells. The concentrations of tramadol and its phase I metabolites were significantly higher in pigmented rat hair while those of phase II metabolites did not showed any consistent significant difference depending on the status of hair pigmentation. ODMT was taken up to a greater extent than ODMT-glucuronide by SK-Mel-28 cells, HaCaT cells and the co-cultured HaCaT cells with SK-Mel-28 cells. Notably, the incorporated level of ODMT was higher in SK-Mel-28 cells than HaCaT cells and the concentration difference of ODMT was significantly larger than that of ODMT-glucuronide. This study clearly demonstrated that hair pigmentation played a role as a facilitating factor for the incorporation of basic compounds and provided insight into the drug incorporation process into hair.
Keimyung Author(s)(Kor)
장정희
Publisher
School of Medicine (의과대학)
Citation
Hyerim Yu et al. (2017). Role of hair pigmentation in drug incorporation into hair. Forensic Science International, 281, 171–175. doi: 10.1016/j.forsciint.2017.11.004
Type
Article
ISSN
0379-0738
Source
https://linkinghub.elsevier.com/retrieve/pii/S0379-0738(17)30460-7
DOI
10.1016/j.forsciint.2017.11.004
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/41454
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Pharmacology (약리학)
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