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Anthocyanins from black soybean seed coat prevent radiationinduced skin fibrosis by downregulating TGF-β and Smad3 expression

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Author(s)
Sang Woo ParkJaehoon ChoiJunhyung KimWoohhyeok JeongJun Sik KimBae Kwon JeongSung Chul ShinJin Hee Kim
Keimyung Author(s)
Choi, Jae HoonKim, Jun HyungKim, Jin Hee
Department
Dept. of Plastic Surgery (성형외과학)
Dept. of Radiation Oncology (방사선종양학)
Journal Title
Arch Dermatol Res.
Issued Date
2018
Volume
310
Issue
5
Keyword
AnthocyaninRadiation-induced fibrosisTGF-β · FibroblastMouse model
Abstract
The aim of this study was to evaluate the protective effects of anthocyanins from the black soybean seed coat against radiation injury in dermal fibroblasts and mouse skin. Dermal fibroblasts treated with 50 and 100 μg/mL anthocyanins were irradiated with single doses of 20 Gy. Cell viability, intracellular reactive oxygen species (ROS) production, and mRNA expression were measured. A total of 60 mice were used for an in vivo study. A dose of 100 μg/mL anthocyanins was administered daily for 5 days before or after radiation therapy. Following irradiation (45 Gy), mice were inspected for gross pathology twice per wk for 8 weeks. At 4 and 8 weeks post-irradiation, dorsal skin was harvested for histopathologic examination and protein isolation. In dermal fibroblasts, treatment with 50 and 100 μg/mL anthocyanins significantly reduced radiation-induced apoptosis at 72 h and intracellular reactive oxygen species generation at 48 h. Furthermore, 100 μg/mL anthocyanins markedly decreased Smad3 mRNA expression and increased Smad7 mRNA expression at 72 h post-irradiation. In mice, treatment with 100 μg/mL anthocyanins resulted in a significant reduction in the level of skin injury, epidermal thickness, and collagen deposition after irradiation. Treatment with 100 μg/mL anthocyanins significantly decreased the number of α-SMA-, TGF-β-, and Smad3-positive cells after irradiation. Our study demonstrated that black soybean anthocyanins inhibited radiation-induced fibrosis by downregulating TGF-β and Smad3 expression. Therefore, anthocyanins may be a safe and effective candidate for the prevention of radiation-induced skin fibrosis.
Keimyung Author(s)(Kor)
최재훈
김준형
김진희
Publisher
School of Medicine (의과대학)
Citation
Sang Woo Park et al. (2018). Anthocyanins from black soybean seed coat prevent radiationinduced skin fibrosis by downregulating TGF-β and Smad3 expression. Arch Dermatol Res., 310(5), 401–412. doi: 10.1007/s00403-018-1827-
Type
Article
ISSN
1432-069X
Source
https://link.springer.com/article/10.1007/s00403-018-1827-7
DOI
10.1007/s00403-018-1827-
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/41699
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Plastic Surgery (성형외과학)
1. School of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학)
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