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Mito-TEMPO improves development competence by reducing superoxide in preimplantation porcine embryos

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Author(s)
Seul-Gi YangHyo-Jin ParkJin-Woo KimJae-Min JungMin-Ji KimHo-Guen JegalIn-Su KimMan-Jong KangGabbine WeeHee-Young YangYun-Han LeeJi-Hae SeoSun-Uk KimDeog-Bon Koo
Keimyung Author(s)
Lee, Yun HanSeo, Ji Hye
Department
Dept. of Molecular Medicine (분자의학)
Dept. of Biochemistry (생화학)
Journal Title
Scientific Reports
Issued Date
2018
Volume
8
Issue
1
Abstract
Mito-TEMPO is a well-known mitochondria-specific superoxide scavenger. However, the effect of Mito-TEMPO on porcine embryo development, to our knowledge, has not been studied yet. In the present study, porcine embryos were classified into two groups (G1 and G2) based on the cytoplasm lipid contents at the zygote stage. The development of blastocysts derived from G2 zygotes was reduced (G2:16.2 ± 7.9% vs G1: 26.5 ± 5.9%; 1.6-fold, p < 0.05) compared to those from G1 zygotes. In G2 embryos, the proportion of TUNEL-positive cells was also higher than that of G1 embryos. Superoxide in G2 embryos was significantly increased compared to that in G1 embryos. Mitochondrial membrane potential and ATP production were lower in G2 embryos than in G1 embryos. Phosphorylation of Drp1 at Ser 616 increased in G1 embryos during the cleavage stages compared to that in the zygote but was not significantly different in G2 embryos. Then, the effects of Mito-TEMPO were investigated in G2 embryos. Blastocyst formation rate (G2: 19.1 ± 5.1% vs G2 + Mito-TEMPO: 28.8 ± 4.0%; 1.5-fold, p < 0.05) and mitochondrial aggregation were recovered after superoxide reduction by Mito-TEMPO treatment. Thus, we showed that Mito-TEMPO improves blastocyst development by superoxide reduction in porcine embryos in vitro.
Keimyung Author(s)(Kor)
이윤한
서지혜
Publisher
School of Medicine (의과대학)
Citation
Seul-Gi Yang et al. (2018). Mito-TEMPO improves development competence by reducing superoxide in preimplantation porcine embryos. Scientific Reports, 8(1), 10130–10130. doi: 10.1038/s41598-018-28497-5
Type
Article
ISSN
2045-2322
Source
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6031607/
DOI
10.1038/s41598-018-28497-5
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/41731
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Biochemistry (생화학)
1. School of Medicine (의과대학) > Dept. of Molecular Medicine (분자의학)
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