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High RNA-binding Motif Protein 3 Expression Is Associated with Improved Clinical Outcomes in Invasive Breast Cancer

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Author(s)
Sun Hee KangJihyoung ChoHasong JeongSun Young Kwon
Keimyung Author(s)
Kang, Sun HeeCho, Ji HyoungKwon, Sun Young
Department
Dept. of Surgery (외과학)
Dept. of Pathology (병리학)
Journal Title
Journal of Breast Cancer
Issued Date
2018
Volume
21
Issue
3
Keyword
Breast neoplasmsImmunohistochemistryPrognosisRNA-binding proteins
Abstract
Purpose:
Expression of RNA-binding motif protein 3 (RBM3) is induced by hypoxia and hypothermia. Recently, high expression of RBM3 was reported to be associated with a good prognosis in colon cancer, prostate cancer, ovarian cancer, and malignant melanoma. Studies on RBM3 in invasive breast carcinoma (IBC), however, are limited.

Methods:
RBM3 expression was examined using a tissue microarray from 361 patients with IBC. Immunohistochemistry was performed for estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (HER2), and Ki-67 to compare the expression of these markers. For scoring of RBM3 expression, NF (nuclear staining fraction)×NI (nuclear staining intensity) was used. The RBM3 expression score was considered indicative of either low (≤4) or high (>4) expression. Western blot analysis was performed on breast cancer cell lines to evaluate RBM3 expression.

Results:
Of the total 361 samples, 240 (66.5%) exhibited high RBM3 expression. High RBM3 expression was significantly associated with positivity for ER (p<0.001), PR (p<0.001), T stage (p<0.001), histologic grade (p<0.001), and % Ki-67 staining (p=0.004). Multivariate analysis revealed that high RBM3 expression was closely associated with prolonged disease-free survival (DFS) (p<0.001) and overall survival (OS) (p<0.001). Western blot analysis revealed reduced RBM3 expression in HCC1954 (HER2-enriched) and BT-20 (basal-like) cells with an aggressive phenotype.

Conclusion:
High nuclear RBM3 expression is strongly associated with a prolonged DFS and OS. Furthermore, RBM3 expression is closely associated with good prognostic markers such as ER and PR in IBC. High nuclear RBM3 expression is, therefore, a critical biomarker of favorable clinical outcomes in IBC.
Keimyung Author(s)(Kor)
강선희
조지형
권선영
Publisher
School of Medicine (의과대학)
Citation
Sun Hee Kang et al. (2018). High RNA-binding Motif Protein 3 Expression Is Associated with Improved Clinical Outcomes in Invasive Breast Cancer. Journal of Breast Cancer, 21(3), 288–296. doi: 10.4048/jbc.2018.21.e34
Type
Article
ISSN
2092-9900
Source
https://ejbc.kr/DOIx.php?id=10.4048/jbc.2018.21.e34
DOI
10.4048/jbc.2018.21.e34
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/41790
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
1. School of Medicine (의과대학) > Dept. of Surgery (외과학)
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