계명대학교 의학도서관 Repository

Circulating exosomal noncoding RNAs as prognostic biomarkers in human hepatocellular carcinoma

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Author(s)
Yu Rim LeeGyeonghwa KimWon Young TakSe Young JangYoung Oh KweonJung Gil ParkHye Won LeeYoung Seok HanJae Min ChunSoo Young ParkKeun Hur
Keimyung Author(s)
Lee, Hye Won
Department
Dept. of Pathology (병리학)
Journal Title
International Journal of Cancer
Issued Date
2018
Volume
144
Issue
6
Keyword
circulating biomarkerexosomemicroRNAlncRNAhepatocellular carcinoma
Abstract
Exosomal noncoding RNAs (ncRNAs) have unique expression profiles reflecting the characteristics of a tumor, and their role in tumor progression and metastasis is emerging. However, the significance of circulating exosomal ncRNAs in the prognosis of hepatocellular carcinoma (HCC) remains to be elucidated. We therefore determined the prognostic significance of circulating exosomal ncRNAs (miRNA-21 and lncRNA-ATB) for human HCC. This prospective study enrolled 79 HCC patients between October 2014 and September 2015. Exosomes were extracted from serum samples using the ExoQuick Exosome Precipitation Solution. To validate the isolation of the exosomes from serum, immunoblotting for exosome markers and characterization of nanoparticle using NanoSight were performed. NcRNAs were isolated from exosomes using the miRNeasy serum/plasma micro kit. Both circulating exosomal miRNA-21 and lncRNA-ATB were related to TNM stage and other prognostic factors, including the T stage and portal vein thrombosis. Multivariate analysis using the Cox regression test identified that both higher miRNA-21 and higher lncRNA-ATB were independent predictors of mortality and disease progression, along with larger tumor size and higher C-reactive protein (all p < 0.05). The overall survival and progression-free survival were significantly lower in patients with higher circulating levels of exosomal miRNA-21 (≥0.09) and lncRNA-ATB (≥0.0016) (log-rank test: p < 0.05). In conclusion, our study has provided strong evidence that circulating exosomal ncRNAs (miRNA-21 and lncRNA-ATB) are novel prognostic markers and therapeutic targets for HCC.
Keimyung Author(s)(Kor)
이혜원
Publisher
School of Medicine (의과대학)
Citation
Yu Rim Lee et al. (2018). Circulating exosomal noncoding RNAs as prognostic biomarkers in human hepatocellular carcinoma. International Journal of Cancer, 144(6), 1444–1452. doi: 10.1002/ijc.31931
Type
Article
ISSN
1097-0215
Source
https://onlinelibrary.wiley.com/doi/full/10.1002/ijc.31931
DOI
10.1002/ijc.31931
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/41809
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
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