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Duodenal-Jejunal Bypass Surgery Stimulates the Expressions of Hepatic Sirtuin1 and 3 and Hypothalamic Sirtuin1

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Author(s)
Eunyoung HaJong Yeon KangKyung Sik ParkYoun Kyoung SeoTae Kyung Ha
Keimyung Author(s)
Ha, Eun Young
Department
Dept. of Biochemistry (생화학)
Journal Title
Journal of Obesity & Metabolic Syndrome
Issued Date
2018
Volume
27
Issue
4
Keyword
Duodenojejunal bypassLife spanSirtuin1Sirtuin3
Abstract
Background:
Sirtuins mediate metabolic responses to nutrient availability and slow aging and accompanying decline in health. This study was designed to assess the expressions of sirtuin1 (SIRT1) and sirtuin3 (SIRT3) in the liver and hypothalamus after duodenal-jejunal bypass (DJB) surgery in rats.

Methods:
A total of 38 rats were randomly assigned to either sham group (n=8) or DJB group (n=30). DJB group was again divided into three groups according to the elapsed time after surgery (10 weeks, DJB10; 16 week, DJB16; 28 week, DJB28). The mRNA and protein expressions of SIRT1 and SIRT3 in the liver and hypothalamus were measured by reverse transcription polymerase chain reaction, Western blot, and immunohistochemistry analyses. NAD+/NADH ratio was also measured.

Results:
We found increased mRNA and protein expression levels of SIRT1 in the liver of DJB16 and DJB28 groups compared with those of sham group. The mRNA and protein expressions of SIRT3 in the liver of DJB group increased proportionally to the elapsed time after DJB surgery. The mRNA expression levels of SIRT1 in the hypothalamus increased in DJB16 and DJB28 groups and protein expression levels of SIRT1 in the hypothalamus increased in DJB10, DBJ16, and DJB28 groups compared with sham group. We observed that mRNA and protein levels of SIRT3 in the hypothalamus of DJB group were not changed.

Conclusion:
This study proves that DJB increases SIRT1 and SIRT3 expressions in the liver and SIRT1 expression in the hypothalamus. These results suggest the possibility of sirtuins being involved in bypass surgery-induced metabolic changes.
Keimyung Author(s)(Kor)
하은영
강종연
박경식
Publisher
School of Medicine (의과대학)
Citation
Eunyoung Ha et al. (2018). Duodenal-Jejunal Bypass Surgery Stimulates the Expressions of Hepatic Sirtuin1 and 3 and Hypothalamic Sirtuin1. Journal of Obesity & Metabolic Syndrome, 27(4), 248–253. doi: 10.7570/jomes.2018.27.4.248
Type
Article
ISSN
2508-7576
Source
http://www.jomes.org/journal/view.html?doi=10.7570/jomes.2018.27.4.248
DOI
10.7570/jomes.2018.27.4.248
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/41860
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Biochemistry (생화학)
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