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The effect of combination therapy on critical-size bone defects using non-activated platelet-rich plasma and adipose-derived stem cells

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Author(s)
Woonhyeok JeongYoung Seok KimTai Suk RohEun Hye KangBok Ki JungIn Sik Yun
Keimyung Author(s)
Jeong, Woon Hyeok
Department
Dept. of Plastic Surgery (성형외과학)
Journal Title
Child's Nervous System
Issued Date
2019
Keyword
Mesenchymal stem cellsPlatelet-rich plasmaOsteogenesisSkull
Abstract
Purpose
Non-activated platelet-rich plasma (nPRP) slowly releases growth factors that induce bone regeneration. Adipose tissue-derived stem cells (ASCs) are also known to induce osteoblast differentiation. In this study, we investigated the combined effect of nPRP and ASC treatment compared with single therapy on bone regeneration.

Methods
Thirty New Zealand white rabbits with 15 × 15 mm2 calvarial defects were randomly divided into four treatment groups: control, nPRP, ASC, or nPRP + ASC groups. For treatment, rabbits received a collagen sponge (Gelfoam®) saturated with 1 ml normal saline (controls), 1 ml non-activated PRP (nPRP group), 2 × 106 ASCs (ASCs group), or 2 × 106 ASCs plus l ml nPRP (nPRP + ASCs group). After 16 weeks, bone volume and new bone surface area were measured, using three-dimensional computed tomography and digital photography. Bone regeneration was also histologically analyzed.

Results
Bone surface area in the nPRP group was significantly higher than both the control and ASC groups (p < 0.001 and p < 0.01, respectively). The percentage of regenerated bone surface area in the nPRP + ASC group was also significantly higher than the corresponding ratios in the control group (p < 0.001). The volume of new bone in the nPRP group was increased compared to the controls (p < 0.05).

Conclusion
Our results demonstrate that slow-releasing growth factors from nPRP did not influence ASC activation in this model of bone healing. PRP activation is important for the success of combination therapy using nPRP and ASCs
Keimyung Author(s)(Kor)
정운혁
Publisher
School of Medicine (의과대학)
Citation
Woonhyeok Jeong et al. (2019). The effect of combination therapy on critical-size bone defects using non-activated platelet-rich plasma and adipose-derived stem cells. Child’s Nervous System. doi: 10.1007/s00381-019-04109-z
Type
Article
ISSN
1433-0350
Source
https://link.springer.com/article/10.1007%2Fs00381-019-04109-z
DOI
10.1007/s00381-019-04109-z
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/41961
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Plastic Surgery (성형외과학)
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