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Recipient-Site Preconditioning With Deferoxamine Increases Fat Graft Survival by Inducing VEGF and Neovascularization in a Rat Model

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Author(s)
Junhyung KimMinwoo ParkWoonhyeok JeongHyoun Wook LeeGeumhong LeeKyung Suk LeeSang Woo ParkJaehoon Choi
Keimyung Author(s)
Kim, Jun HyungJeong, Woon HyeokChoi, Jae Hoon
Department
Dept. of Plastic Surgery (성형외과학)
Journal Title
Plastic and reconstructive surgery
Issued Date
2019
Volume
144
Issue
4
Abstract
Background:
The authors hypothesize that ischemic preconditioning of the recipient site with deferoxamine will increase fat graft survival by enhancing angiogenesis in a rat model.

Methods:
Cell viability, tube formation, and mRNA expression were measured in human umbilical vein endothelial cells treated with deferoxamine. A total of 36 rats were then used for an in vivo study. A dose of 100 mg/kg of deferoxamine was injected subcutaneously into the rat scalp every other day for five treatments. On the day after the final injection, the scalp skin was harvested from half the animals to evaluate the effects of deferoxamine on the recipient site. In the remaining animals, inguinal fat tissue was transplanted to the scalp. Eight weeks after transplantation, the grafts were harvested to evaluate the effects of deferoxamine preconditioning on fat graft survival.

Results:
In human umbilical vein endothelial cells, treatment with a deferoxamine concentration higher than 400 μM decreased cell viability compared with the control (p = 0.002). Treatment with 100 and 200 μM deferoxamine increased endothelial tube formation (p = 0.001) and mRNA levels of angiogenesis-related factors (p = 0.02). Rat scalps treated with deferoxamine exhibited increased capillary neoformation (p = 0.001) and vascular endothelial growth factor protein expression (p = 0.024) compared with controls. Fat graft volume retention, capillary density (p < 0.001), and adipocyte viability (p < 0.001) in the grafted fat increased when the recipient site was preconditioned with deferoxamine.

Conclusion:
This study demonstrated that recipient site preconditioning with deferoxamine increases fat graft survival by inducing vascular endothelial growth factor and neovascularization.
Keimyung Author(s)(Kor)
김준형
정운혁
최재훈
Publisher
School of Medicine (의과대학)
Citation
Junhyung Kim et al. (2019). Recipient-Site Preconditioning With Deferoxamine Increases Fat Graft Survival by Inducing VEGF and Neovascularization in a Rat Model. Plastic and reconstructive surgery, 144(4), 619e–629e. doi: 10.1097/PRS.0000000000006036
Type
Article
ISSN
1529-4242
Source
https://journals.lww.com/plasreconsurg/fulltext/2019/10000/Recipient_Site_Preconditioning_with_Deferoxamine.23.aspx
DOI
10.1097/PRS.0000000000006036
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/42407
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Plastic Surgery (성형외과학)
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