계명대학교 의학도서관 Repository

Cyclosporine A Eyedrops With Self-Nanoemulsifying Drug Delivery Systems Have Improved Physicochemical Properties and Efficacy Against Dry Eye Disease in a Murine Dry Eye Model

Metadata Downloads
Author(s)
Seung Pil BangChang Yeor YeonNirpesh AdhikariSanjiv NeupaneHarim KimDong Cheol LeeMyeong Jin SonHyun Gyo LeeJae-Young KimJong Hwa Jun
Keimyung Author(s)
Lee, Dong CheolJun, Jong Hwa
Department
Dept. of Ophthalmology (안과학)
Journal Title
PloS one
Issued Date
2019
Volume
14
Issue
11
Abstract
Purpose:
We aimed to compare the physicochemical properties and in vivo efficacy of commercially available nanoemulsion cyclosporine A (CsA) eyedrops in benzalkonium chloride (BAC)-induced dry eye disease (DED).

Methods:
Particle size analysis was performed on conventional 0.05% CsA (Restasis, C-CsA) and two new types of 0.05% CsA eyedrops based on a self-nanoemulsifying drug delivery system (SNEDDS, SNEDDS-N and -T). Turbidometry, pH measurements and instability indices of each CsA solution were measured. DED was induced with BAC, and animals were treated with vehicle or CsA preparations. Tear volume and fluorescein staining scores were evaluated on days 7 and 14. Eyes were enucleated and subjected to IHC, TUNEL staining, periodic acid-Schiff (PAS) staining, real-time PCR and western blotting.

Results:
Both SNEDDSs had lower and more uniform particle size distribution than C-CsA, and a similar optical density to phosphate-buffered saline and stable pH, in contrast to the high turbidity and unstable pH of C-CsA. Aqueous tear volume and fluorescein staining scores were improved in C-CsA- and SNEDDS-treated mice. Numbers of PAS-positive goblet cells and levels of inflammatory mediators were decreased by both C-CsA and SNEDDS, although SNEDDS resolved inflammation more effectively than C-CsA.

Conclusions:
Cyclosporine A eyedrops with SNEDDS have improved physicochemical properties and treatment efficacy in BAC-induced DED.
Keimyung Author(s)(Kor)
이동철
전종화
Publisher
School of Medicine (의과대학)
Citation
Seung Pil Bang et al. (2019). Cyclosporine A Eyedrops With Self-Nanoemulsifying Drug Delivery Systems Have Improved Physicochemical Properties and Efficacy Against Dry Eye Disease in a Murine Dry Eye Model. PloS one, 14(11), e0224805–e0224805. doi: 10.1371/journal.pone.0224805
Type
Article
ISSN
1932-6203
Source
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0224805
DOI
10.1371/journal.pone.0224805
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/42408
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Ophthalmology (안과학)
공개 및 라이선스
  • 공개 구분공개
파일 목록

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.