Influence of Human PapillomaVirus Infection Detected by Cervical Swap on In-vitro Fertilization Outcomes

Abstract

Human papilloma virus (HPV) infection had been suspected of harmful effect on reproduction by increasing blastocyst apoptosis and reducing the endometrial implantation of trophoblastic cells. However, the available evidence is conflicting concerning the impact of HPV in female reproductive system on the outcome of in-vitro fertilization (IVF). The aim of study was to evaluate the IVF outcomes regarding the cervical HPV infection. Retrospective case-control study conducted in women suffered IVF cycle. All women were evaluated HPV infection using PCR by cervical swap before the initiation of IVF cycle. One hundred thirty six cycles were evaluated. The harvested oocytes number, top quality embryo number, fertilization rate, implantation rate, clinical pregnancy rate, live birth rate and miscarriage rate were analyzed depend on HPV infection. Mean age of enrolled women was 35.20±4.79 years-old, mean parity was 0.17±0.57, and mean number of miscarriage was 0.81±1.49). Basal FSH was 5.42±2.59 IU/L and AMH level was 5.07±11.01 ng/mL, which was not different between two groups. Mean harvested oocytes was 6.36±4.32 in HPV infected women compared with 8.63±5.95 in women without infection, statistically insignificant. Top quality embryos of each group were not different (2.67±0.82, 2.53±1.53 separately). In women with HPV infection, ongoing pregnancy rate (27.3%) and live birth rate (18.2%) were similar with women without infection (23.7%, 14.0%). And also, fertilization rate and implantation rate were similar. Miscarriage rate of each group was 33.3%, and 40.7%, which was statistically insignificant. The HPV infection in women did not make the adverse influence on IVF outcomes.