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Viral Load Kinetics of SARS-CoV-2 Infection in Saliva in Korean Patients: a Prospective Multi-center Comparative Study

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Author(s)
Seong Eun KimJi Yeon LeeAhrang LeeSoosung KimKyung-Hwa ParkSook-In JungSeung-Ji KangTae Hoon OhUh Jin KimSeung Yeob LeeSeung-Jung KeeHee-Chang Jang
Keimyung Author(s)
Lee, Ji Yeon
Department
Dept. of Internal Medicine (내과학)
Journal Title
Journal of Korean Medical Science
Issued Date
2020
Volume
35
Issue
31
Keyword
SARS-CoV-2 PCRSalivaNasopharyngeal SwabOropharyngeal Swab
Abstract
Background:
This study was performed to compare the viral load and kinetics of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) in saliva with those in standard nasopharyngeal/oropharyngeal (NP/OP) swabs.

Methods:
Fifteen patients with SARS-CoV-2 infection from four hospitals were prospectively enrolled and matched samples of nasopharyngeal/oropharyngeal swabs and saliva were collected at Day 1 of admission and every other day till consequently negative for two times. Real-time reverse transcription polymerase chain reaction (rRT-PCR) was performed to detect the envelope (E) and RNA-dependent RNA polymerase (RdRP) genes.

Results:
The cycle threshold values of saliva were comparable to those of NP/OP swabs overall (P = 0.720, Mann–Whitney U test). However, the overall sensitivity of rRT-PCR using saliva was 64% (34/53), which is lower than the 77% (41/53) using NP/OP swabs. The sensitivity of rRT-PCR using saliva was especially lower in early stage of symptom onset (1–5 days; 8/15; 53%) and in patients who did not have sputum (12/22; 55%).

Conclusion:
Saliva sample itself is not appropriate for initial diagnosis of coronavirus disease 2019 (COVID-19) to replace NP/OP swabs, especially for the person who does not produce sputum. COVID-19 cannot be excluded when the test using saliva is negative, and it is necessary to retest using NP/OP swabs.
Keimyung Author(s)(Kor)
이지연
Publisher
School of Medicine (의과대학)
Citation
Seong Eun Kim et al. (2020). Viral Load Kinetics of SARS-CoV-2 Infection in Saliva in Korean Patients: a Prospective Multi-center Comparative Study. Journal of Korean Medical Science, 35(31), e287. doi: 10.3346/jkms.2020.35.e287
Type
Article
ISSN
1598-6357
Source
https://jkms.org/search.php?where=aview&id=10.3346/jkms.2020.35.e287&code=0063JKMS&vmode=FULL
DOI
10.3346/jkms.2020.35.e287
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/42868
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
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