계명대학교 의학도서관 Repository

Transcriptional regulation of Salmochelin glucosyltransferase by Fur in Salmonella

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Author(s)
Daejin LimKyeongmin KimMiryoung SongJae-Ho JeongJeong Ho ChangSang Ryong KimChang-won HongSeung-Soon ImSeung-Hwan ParkJe Chul LeeMinsang Shin
Keimyung Author(s)
Im, Seung Soon
Department
Dept. of Physiology (생리학)
Journal Title
Biochemical and biophysical research communications
Issued Date
2020
Volume
529
Issue
1
Keyword
FurSalmochelin glucosyltransferaseRepressorSalmonella
Abstract
Pathogenic bacteria acquire the acquisition of iron from the host to ensure their survival. Salmonella spp. utilizes siderophores, including salmochelin, for high affinity aggressive import of iron. Although the iroBCDEN operon is reportedly responsible for the production and the transport of salmochelin, the molecular mechanisms underlying the regulation of its gene expression have not yet been characterized. Here, we analyzed the expression pattern of iroB using the lacZY transcriptional reporter system and determined the transcription start site in response to iron availability using primer extension analysis. We further examined the regulation of iroB expression by the ferric uptake regulator (Fur), a key regulatory protein involved in the maintenance of iron homeostasis in various bacteria, including Salmonella. Using sequence analysis followed by a gel shift assay, we verified that the Fur box lies within the promoter region of iroBCDE. The Fur box contained the consensus sequence (GATATTGGTAATTATTATC) and overlapped with the 10-element region. The expression of iroB was repressed by Fur in the presence of iron, as determined using an in vitro transcription assay. Therefore, we found that the iron acquisition system is regulated in a Fur-dependent manner in Salmonella.
Keimyung Author(s)(Kor)
임승순
Publisher
School of Medicine (의과대학)
Citation
Daejin Lim et al. (2020). Transcriptional regulation of Salmochelin glucosyltransferase by Fur in Salmonella. Biochemical and biophysical research communications, 529(1), 70–76. doi: 10.1016/j.bbrc.2020.06.009
Type
Article
ISSN
1090-2104
Source
https://www.sciencedirect.com/science/article/pii/S0006291X20312031
DOI
10.1016/j.bbrc.2020.06.009
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/42962
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Physiology (생리학)
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