계명대학교 의학도서관 Repository

Flexible and highly ordered nanopillar electrochemical sensor for sensitive insulin evaluation

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Author(s)
Yoo Min ParkYoung Sun ChoiHye-Rim LeeYun Seok HeoNam Ho BaeTae Jae LeeSeok Jae Lee
Keimyung Author(s)
Heo, Yun Seok
Department
Dept. of Biomedical Engineering (의용공학과)
Journal Title
Biosensors & bioelectronics
Issued Date
2020
Volume
161
Abstract
In line with growing interest in obesity management, there has been an increase in the amount of research focused on highly sensitive analysis systems for a small number of biomarers. In this paper, we introduce the highly ordered nanopillar electrode, featuring a high aspect ratio surface area that enables enhanced electron transfer. For fabrication of the flexible electrode, gold was evaporated by electronic beam lithography on polyurethane (PU), which has high flexibility. The fabricated nanopillar is 500 nm in diameter and 1500 nm in height. Based on the highly ordered nanostructure electrode, insulin was selected as a biomarker to monitor the insulin resistance associated with obesity. To effectively analyze the insulin, the self-assembled monolayer chemical was used to introduce the enzyme catalysis-based electrochemical immunoassay, leading to the analysis of the insulin concentration range from 0.1 to 1.0 ng/mL in the real sample. The square wave voltammetry principle was used to measure HRP-based electrochemical signal both electrochemically and quantitatively. Based on the nanostructural properties of significant electrochemical behavior, we successfully analyzed insulin in the plasma sample with high sensitivity (LOD of 0.1 ng/mL) and with high reproducibility (<10%). The obtained sensitivity of nanopillar electrode is approximately 10 times (1020%) greater than that of commercial electrode. The results demonstrated that the nanopillar electrode is suitable for precise and sensitive analysis of low-level biomolecules in medical and commercial fields.
Keimyung Author(s)(Kor)
허윤석
Publisher
School of Medicine (의과대학)
Citation
Yoo Min Park et al. (2020). Flexible and highly ordered nanopillar electrochemical sensor for sensitive insulin evaluation. Biosensors & bioelectronics, 161, 112252. doi: 10.1016/j.bios.2020.112252
Type
Article
ISSN
1873-4235
Source
https://www.sciencedirect.com/science/article/abs/pii/S0956566320302487
DOI
10.1016/j.bios.2020.112252
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/42968
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Biomedical Engineering (의용공학과)
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