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Importance of tear volume for positivity of tear matrix metalloproteinase-9 immunoassay

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Author(s)
Jong Hwa JunYou Hyun LeeMyeong Jin SonHarim Kim
Keimyung Author(s)
Jun, Jong HwaLee, You Hyun
Department
Dept. of Ophthalmology (안과학)
Journal Title
PLoS One
Issued Date
2020
Volume
15
Issue
7
Abstract
The tear matrix metalloproteinase-9 (MMP-9) immunoassay (Inflammadry) exhibits variable results in dry eye (DE) patients. We investigated if the tear volume in DE patients affects the results of MMP-9 immunoassay in clinical and in vitro settings. This cross-sectional study enrolled 188 eyes of 188 DE patients. The clinical symptoms and signs of DE were assessed using the Ocular Surface Disease Index and visual analog scale, strip meniscometry, tear break-up time, and tear meniscus height (TMH), area (TMA), and depth (TMD) using swept-source optical coherence tomography and corneal and conjunctival staining scores. For quantitative evaluation, the bands produced by the InflammaDry test were analyzed with ImageJ. DE subjects were grouped according to MMP-9 positivity and TMH. The InflammaDry-positive group showed greater TMH, TMA, and TMD than the MMP-9-negative group (p < 0.05). InflammaDry test band density in the high TMH group was significantly greater than that in the low and normal TMH groups (p < 0.05). InflammaDry test band density correlated positively with TMH, TMA, and TMD (all p < 0.05). InflammaDry test results were influenced by tear volume. Low tear volume in aqueous tear-deficient DE may induce false-negative results, and reflex tearing during the test may induce false-positive results.
Keimyung Author(s)(Kor)
전종화
이유현
Publisher
School of Medicine (의과대학)
Citation
Jong Hwa Jun et al. (2020). Importance of tear volume for positivity of tear matrix metalloproteinase-9 immunoassay. PLoS One, 15(7), e0235408. doi: 10.1371/journal.pone.0235408
Type
Article
ISSN
1932-6203
Source
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0235408
DOI
10.1371/journal.pone.0235408
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/43277
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Ophthalmology (안과학)
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