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Alpha-tocopherol exerts protective function against the mucotoxicity of particulate matter in amphibian and human goblet cells

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Author(s)
Hee-Sun YangHyo Jung SimHanna ChoWoo Young BangHa Eun KimTaeg Kyu KwonTaejoon KwonTae Joo Park
Keimyung Author(s)
Kwon, Taeg Kyu
Department
Dept. of Immunology (면역학)
Journal Title
Scientific Reports
Issued Date
2020
Volume
10
Issue
1
Abstract
Exposure to particulate matter (PM) in ambient air is known to increase the risk of cardiovascular disorders and mortality. The cytotoxicity of PM is mainly due to the abnormal increase of reactive oxygen species (ROS), which damage cellular components such as DNA, RNA, and proteins. The correlation between PM exposure and human disorders, including mortality, is based on long-term exposure. In this study we have investigated acute responses of mucus-secreting goblet cells upon exposure to PM derived from a heavy diesel engine. To this end, we employed the mucociliary epithelium of amphibian embryos and human Calu-3 cells to examine PM mucotoxicity. Our data suggest that acute exposure to PM significantly impairs mucus secretion and results in the accumulation of mucus vesicles in the cytoplasm of goblet cells. RNA-seq analysis revealed that acute responses to PM exposure significantly altered gene expression patterns; however, known regulators of mucus production and the secretory pathway were not significantly altered. Interestingly, pretreatment with α-tocopherol nearly recovered the hyposecretion of mucus from both amphibian and human goblet cells. We believe this study demonstrates the mucotoxicity of PM and the protective function of α-tocopherol on mucotoxicity caused by acute PM exposure from heavy diesel engines.
Keimyung Author(s)(Kor)
권택규
Publisher
School of Medicine (의과대학)
Citation
Hee-Sun Yang et al. (2020). Alpha-tocopherol exerts protective function against the mucotoxicity of particulate matter in amphibian and human goblet cells. Scientific Reports, 10(1), 6224. doi: 10.1038/s41598-020-63085-6
Type
Article
ISSN
2045-2322
Source
https://www.nature.com/articles/s41598-020-63085-6
DOI
10.1038/s41598-020-63085-6
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/43291
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
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