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Regulation of Ras homolog family member G by microRNA-124 regulates proliferation and migration of human retinal pigment epithelial cells

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Author(s)
Jong Hwa JunMyeong-Jin SonHyun-Gyo LeeKyu Young ShimWon-Ki BaekJae-Young KimChoun-Ki Joo
Keimyung Author(s)
Jun, Jong HwaBaek, Won Ki
Department
Dept. of Ophthalmology (안과학)
Dept. of Microbiology (미생물학)
Journal Title
Scientific Reports
Issued Date
2020
Volume
10
Issue
1
Abstract
Uncontrolled retinal pigment epithelial (RPE) cell proliferation/migration contribute to the pathological tractional membrane development in proliferative vitreoretinopathy. Recent studies reported that microRNA (miR)-124 controls various cellular functions via the direct targeting of small Ras homolog family member G (RHOG). Therefore, we investigated the role of the neuron-specific miR-124 and RHOG in RPE cell proliferation/migration. Alterations in miR-124 and RhoG expression, as per cell confluence were evaluated through quantitative real-time PCR and western blotting, respectively. After transfection with miR-124, we quantified RPE cell viability and migration and observed cell polarization and lamellipodia protrusions. We evaluated the expression of RHOG/RAC1 pathway molecules in miR-124-transfected RPE cells. Endogenous miR-124 expression increased proportionally to RPE cell density, but decreased after 100% confluence. Overexpression of miR-124 decreased cell viability and migration, BrdU incorporation, and Ki-67 expression. Inhibition of endogenous miR-124 expression promoted RPE cell migration. Transfection with miR-124 reduced cell polarization, lamellipodia protrusion, and RHOG mRNA 3' untranslated region luciferase activity. Like miR-124 overexpression, RhoG knockdown decreased RPE cell viability, wound healing, and migration, and altered the expression of cell cycle regulators. These results suggest that miR-124 could be a therapeutic target to alleviate fibrovascular proliferation in retinal diseases by regulating RPE proliferation/migration via RHOG.
Keimyung Author(s)(Kor)
전종화
손명진
이현교
심규영
백원기
Publisher
School of Medicine (의과대학)
Citation
Jong Hwa Jun et al. (2020). Regulation of Ras homolog family member G by microRNA-124 regulates proliferation and migration of human retinal pigment epithelial cells. Scientific Reports, 10(1), 15420. doi: 10.1038/s41598-020-72360-5
Type
Article
ISSN
2045-2322
Source
https://www.nature.com/articles/s41598-020-72360-5
DOI
10.1038/s41598-020-72360-5
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/43301
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
1. School of Medicine (의과대학) > Dept. of Ophthalmology (안과학)
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