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Interleukin 1β Up-Regulates mRNA Expression of Inducible Nitric Oxide Synthase in 3T3-L1 Preadipocytes: Role of JAKs/STATs, PKCs, and Src

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Author(s)
박유경왕사이니장병철
Keimyung Author(s)
Jang, Byeong Churl
Department
Dept. of Molecular Medicine (분자의학)
Journal Title
Keimyung Med J
Issued Date
2021
Volume
40
Issue
1
Keyword
3T3-L1 CellsApha-cyano-(3,4-dihydroxy)-N-benzylcinnamideInducible nitric oxide synthaseGO6976Interleukin-1β
Abstract
Recent evidence suggests obesity as a low or systemic chronic inflammation. (Pre)adipocytes in the adipose tissue (AT) express and secrete a variety of cytokines and adipokines. Inducible nitric oxide synthase (iNOS) is an inflammatory enzyme involved in the production of NO. Until now, the inducer(s) of iNOS expression in (pre)adipocytes remains unclear. In this study, we investigated the effects of proinflammatory cytokines [(interleukin-1β (IL-1β), IL-10, IL-12, interferon-γ (IFN-γ)], adipokines [(retinol-binding protein 4 (RBP4), adiponectin, leptin, and resistin)], and lipopolysaccharide (LPS), a bacterial cell wall component, on the expression of iNOS in 3T3-L1 preadipocytes. Notably, treatment with IL-1β at 20 ng/mL for 4 h markedly increased iNOS mRNA expression in 3T3-L1 preadipocytes, but that with IL-10 (10 ng/mL), IL-12 (5 ng/mL), IFN-γ (10 ng/mL), RBP4 (5 ug/mL), adiponectin (100 ng/mL), leptin (100 ng/mL), and resistin (100 ng/mL), and LPS (1 ug/mL) for 4 h had little or no effect on it. Results of dose-response and time-course experiments confirmed the ability of IL-1β at 20 ng/mL for 4 h to maximally induce iNOS mRNA expression in 3T3-L1 preadipocytes. Importantly, pharmacological inhibition studies demonstrated that treatment with AG490 [(an inhibitor of Janus-activated kinases (JAKs) and signal transducer and activator of transcription proteins (STATs))], GO6976 (an inhibitor of PKCs), or PP1 (an Src kinase inhibitor) suppressed IL-1β-induced iNOS mRNA expression in 3T3-L1 preadipocytes, pointing out the involvement of JAKs/STATs, PKCs, and Src in the process. This work advocates that IL-1β is a major and strong inducer of iNOS expression in 3T3-L1 preadipocytes.
Keimyung Author(s)(Kor)
장병철
Citation
박유경. (2021). Interleukin 1β Up-Regulates mRNA Expression of Inducible Nitric Oxide Synthase in 3T3-L1 Preadipocytes: Role of JAKs/STATs, PKCs, and Src. Keimyung Med J, 40(1), 1-8. doi: 10.46308/kmj.2021.00108
Type
Journal
Source
https://www.e-kmj.org/journal/view.php?number=2241
DOI
10.46308/kmj.2021.00108
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/43535
Appears in Collections:
2. Keimyung Medical Journal (계명의대 학술지) > 2021
1. School of Medicine (의과대학) > Dept. of Molecular Medicine (분자의학)
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