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Freeze-dried bovine amniotic membrane as a cell delivery scaffold in a porcine model of radiation-induced chronic wounds

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Author(s)
Daemyung OhDaegu SonJinhee KimSun-Young Kwon
Keimyung Author(s)
Son, Dae GuKim, Jin HeeKwon, Sun Young
Department
Dept. of Plastic Surgery (성형외과학)
Dept. of Radiation Oncology (방사선종양학)
Dept. of Pathology (병리학)
Journal Title
Arch Plast Surg
Issued Date
2021
Volume
48
Issue
4
Keyword
Amniotic membraneCell therapyTissue scaffoldWound healingExperimental animal model
Abstract
Background:
Locoregional stem cell delivery is very important for increasing the efficiency of cell therapy. Amnisite BA (Amnisite) is a freeze-dried amniotic membrane harvested from bovine placenta. The objective of this study was to investigate the retention of cells of the stromal vascular fraction (SVF) on Amnisite and to determine the effects of cell-loaded Amnisite in a porcine radiation-induced chronic wound model.

Methods:
Initially, experiments were conducted to find the most suitable hydration and incubation conditions for the attachment of SVF cells extracted from pig fat to Amnisite. Before seeding, SVFs were labeled with PKH67. The SVF cell-loaded Amnisite (group S), Amnisite only (group A), and polyurethane foam (group C) were applied to treat radiation-induced chronic wounds in a porcine model. Biopsy was performed at 10, 14, and 21 days post-operation for histological analysis.

Results:
Retaining the SVF on Amnisite required 30 minutes for hydration and 1 hour for incubation. A PKH67 fluorescence study showed that Amnisite successfully delivered the SVF to the wounds. In histological analysis, group S showed increased re-epithelialization and revascularization with decreased inflammation at 10 days post-operation.

Conclusions:
SVFs had acceptable adherence on hydrated Amnisite, with successful cell delivery to a radiation-induced chronic wound model.
Keimyung Author(s)(Kor)
손대구
김진희
권선영
Publisher
School of Medicine (의과대학)
Citation
Daemyung Oh et al. (2021). Freeze-dried bovine amniotic membrane as a cell delivery scaffold in a porcine model of radiation-induced chronic wounds. Arch Plast Surg, 48(4), 448–456. doi: 10.5999/aps.2020.00997
Type
Article
ISSN
2234-6171
Source
https://e-aps.org/journal/view.php?year=2021&vol=48&spage=448
DOI
10.5999/aps.2020.00997
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/43671
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
1. School of Medicine (의과대학) > Dept. of Plastic Surgery (성형외과학)
1. School of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학)
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