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Farrerol Induces Cancer Cell Death via ERK Activation in SKOV3 Cells and Attenuates TNF-α-Mediated Lipolysis

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Author(s)
Jongbeom ChaeJin Soo Kimseok tae ChoiSeul Gi LeeOyindamola Vivian OjulariYoung Jin KangTaeg Kyu KwonJu-Ock Nam
Keimyung Author(s)
Kwon, Taeg Kyu
Department
Dept. of Immunology (면역학)
Journal Title
Int J Mol Sci
Issued Date
2021
Volume
22
Issue
17
Keyword
antitumor effectadipogenesiscachexiacell cycle arrestfarrerolovarian cancer
Abstract
Farrerol (FA) is a flavanone isolated from the Chinese herbal medicine "Man-shan-hong" (Rhododendron dauricum L.). In the present study, FA decreased the viability of SKOV3 cells in a dose- and time-dependent manner, and it induced G2/M cell cycle arrest and cell apoptosis. Cell cycle distribution analysis via flow cytometry showed that FA decreased G1 populations and increased G2/M populations in SKOV3 cells. Additionally, Western blotting confirmed an increase in the expression level of proteins involved in the cell cycle, e.g., CDK and cyclins. FA-induced apoptosis in SKOV3 cells was also investigated using a TUNEL assay, and increased expression levels of proapoptotic factors, including Caspase-3 and poly ADP ribose polymerase (PARP), through the Extracellular signal-regulated kinase (ERK)/MAPK pathway were investigated. Proinflammatory cytokines (e.g., IL-6, TNF-α, and IL-1) have been identified as a driver of the pathological mechanisms underlying involuntary weight loss and impaired physical function, i.e., cachexia, during cancer; in the present study, we showed that farrerol attenuates TNF-α-induced lipolysis and increases adipogenic differentiation in 3T3-L1 cells. Thus, farrerol could potentially be used as an anticancer agent or anticachetic drug.
Keimyung Author(s)(Kor)
권택규
Publisher
School of Medicine (의과대학)
Citation
Jongbeom Chae et al. (2021). Farrerol Induces Cancer Cell Death via ERK Activation in SKOV3 Cells and Attenuates TNF-α-Mediated Lipolysis. Int J Mol Sci, 22(17), 9400. doi: 10.3390/ijms22179400
Type
Article
ISSN
1422-0067
Source
https://www.mdpi.com/1422-0067/22/17/9400
DOI
10.3390/ijms22179400
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/43953
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
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