계명대학교 의학도서관 Repository

Serial administration of rhBMP-2 and alendronate enhances the differentiation of osteoblasts

Metadata Downloads
Author(s)
Sang-Hyon KimHye-Jung ChoiDae Sung YoonChang-Nam Son
Keimyung Author(s)
Kim, Sang HyonSon, Chang Nam
Department
Dept. of Internal Medicine (내과학)
Journal Title
Int J Rheum Dis
Issued Date
2021
Volume
24
Issue
10
Keyword
alendronatebone morphogenetic protein-2osteogenesis
Abstract
Aim:
The incorporation of growth factors is an effective strategy to accelerate bone induction. Bone morphogenetic protein-2 (BMP-2) promotes osteoblast differentiation and induces bone formation. Alendronate (ALN) is an osteoclast deactivation drug. We investigated the effect of serial administration of recombinant human BMP-2 (rhBMP-2) and ALN on osteoblast differentiation.

Methods:
The effect of serial administration of rhBMP-2 (0-150 ng/mL) and ALN (0-15 µmol/L) on the viability and differentiation of a clonal murine calvarial cell line, MC3T3-E1, was evaluated at various concentrations and for different periods. The Cell Counting Kit-8 assay was used to assess cell viability. The alkaline phosphatase activity was evaluated as an indicator of osteogenic differentiation. The expression levels of runt domain-containing transcription factor 2 (Runx2) and osteopontin (OPN) were analyzed by real-time polymerase chain reaction and western blotting. Statistical analyses were performed using Student's t test.

Results:
The serial treatment with rhBMP-2 and ALN increased the expression of the differentiation-related factors Runx2 and OPN, as well as the differentiation ability of osteoblasts compared with individual or simultaneous treatment. The osteoblasts treated with rhBMP-2 followed by ALN showed the highest differentiation. The degree of differentiation in the group treated with rhBMP-2 for 7 days followed by ALN for 3 days was increased by 1.5 times compared with that of the group treated with rhBMP-2 alone (P < .01).

Conclusion:
These findings indicate that the serial administration of rhBMP-2 and ALN may exert osteogenic effects on osteoblastic cells via the upregulation of Runx2 and OPN.
Keimyung Author(s)(Kor)
김상현
손창남
Publisher
School of Medicine (의과대학)
Citation
Sang-Hyon Kim et al. (2021). Serial administration of rhBMP-2 and alendronate enhances the differentiation of osteoblasts. Int J Rheum Dis, 24(10), 1266–1272. doi: 10.1111/1756-185X.14189
Type
Article
ISSN
1756-185X
Source
https://onlinelibrary.wiley.com/doi/10.1111/1756-185X.14189
DOI
10.1111/1756-185X.14189
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/43955
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
공개 및 라이선스
  • 공개 구분공개
  • 엠바고Forever
파일 목록
  • 관련 파일이 존재하지 않습니다.

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.