계명대학교 의학도서관 Repository

Liquid Biopsy from Bile-Circulating Tumor DNA in Patients with Biliary Tract Cancer

Metadata Downloads
Affiliated Author(s)
안근수김태석김용훈조광범신동우백원기서성일장병철강구정
Alternative Author(s)
Ahn, Keun SooKim, Tae SeokKim, Yong HoonCho, Kwang BumShin, Dong WooBaek, Won KiSuh, Seong IlJang, Byeong ChurlKang, Koo Jeong
Journal Title
Cancers (Basel)
ISSN
2072-6694
Issued Date
2021
Keyword
circulating tumor DNA (ctDNA)KRASbiliary tract cancer (BTC)droplet digital polymerase chain reaction (ddPCR)transcriptomic sequencing
Abstract
Although liquid biopsy of blood is useful for cancer diagnosis and prediction of prognosis, diagnostic and prognostic value of ctDNA in bile fluid for BTCs are not clear yet. To determine whether liquid biopsy for circulating tumor DNA (ctDNA) can replace tissue biopsy when assessing somatic mutations in biliary tract cancers (BTCs). Bile samples were obtained from 42 patients with BTC. Matched formalin-fixed paraffin-embedded (FFPE) samples were obtained from 20 of these patients and matched plasma samples from 16 of them. Droplet digital PCR (ddPCR) was used for detection KRAS somatic mutation. KRAS mutations were identified in the bile ctDNA of 20 of 42 (48%) patients. Patients with mutant KRAS showed significantly worse survival than those with wild-type KRAS (2-year survival rates: 0% vs. 55.5%, respectively; p = 0.018). There was 80.0% mutational concordance between the paired bile ctDNA and FFPE samples, and 42.9% between the plasma and FFPE samples. On transcriptomic sequencing of one set of paired bile and FFPE samples, expression level of KRAS-associated signaling oncogenes in the bile and tissue samples showed a strong positive correlation (r = 0.991, p < 0.001). Liquid biopsy of bile reliably detect mutational variants within the bile ctDNA of BTC patients. These results suggest that bile is an effective biopsy fluid for ctDNA analysis.
Authorize & License
  • Authorize공개
Files in This Item:

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.