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Association of tear matrix metalloproteinase 9 immunoassay with signs and symptoms of dry eye disease: A cross-sectional study using qualitative, semiquantitative, and quantitative strategies

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Author(s)
You Hyun LeeSeung-Pil BangKyu-Young ShimMyung-Jin SonHarim KimJong Hwa Jun
Keimyung Author(s)
Lee, You HyunJun, Jong Hwa
Department
Dept. of Ophthalmology (안과학)
Journal Title
PLoS One
Issued Date
2021
Volume
16
Issue
10
Abstract
Purpose:
This study aimed to analyze the association of tear matrix metalloproteinase 9 (MMP-9) immunoassay with the severity of dry eye (DE) signs and symptoms through qualitative, semiquantitative, and quantitative evaluations of immunoassay band.

Materials and methods:
This cross-sectional study enrolled 320 eyes of 320 patients. The clinical signs of DE were assessed using the Ocular Surface Disorder Index (OSDI) score, visual analogue scale (VAS), tear breakup time (tBUT), tear volume evaluation by tear meniscometry, and staining scores of the cornea and conjunctiva by the Oxford grading scheme. The tear MMP-9 immunoassay results were interpreted using qualitative (positive or negative), semi-quantitative (reagent band density on a four-point scale: 0 = negative; 1 = weakly positive; 2 = moderately positive; 3 = strongly positive), and quantitative (ratio of reagent band density to control band density) indicators.

Results:
Positive MMP-9 immunoassay results were significantly related to shorter tBUT, tBUT ≤3 seconds, higher corneal staining score, corneal staining score ≥2, and conjunctival staining score ≥2. The semi-quantitative results of the MMP-9 immunoassay were positively correlated with higher corneal staining score (r = 0.122, p = 0.029) and negatively correlated with tBUT (r = -0.125, p = 0.025). However, in the quantitative analysis, none of the DE signs or symptoms were correlated to the band density of the MMP-9 immunoassay.

Conclusions:
The positive MMP-9 immunoassay results were related to the severity of ocular signs of DE. However, using quantitative measures of the MMP-9 immunoassay to assess the clinical severity of DE requires further investigation.
Keimyung Author(s)(Kor)
이유현
전종화
Publisher
School of Medicine (의과대학)
Citation
You Hyun Lee et al. (2021). Association of tear matrix metalloproteinase 9 immunoassay with signs and symptoms of dry eye disease: A cross-sectional study using qualitative, semiquantitative, and quantitative strategies. PLoS One, 16(10), e0258203. doi: 10.1371/journal.pone.0258203
Type
Article
ISSN
1932-6203
Source
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0258203
DOI
10.1371/journal.pone.0258203
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/44063
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Ophthalmology (안과학)
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