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Weight Change Alters the Small RNA Profile of Urinary Extracellular Vesicles in Obesity

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Author(s)
Dughyun ChoiSewon KimJeyoung WooHaekyung LeeHyoungnae KimJin Seok JeonHyunjin NohDong Cheol HanSang Hyun KimHo Chan ChoJong-Soo ChoiYun-Ui BaeSoon Hyo Kwon
Keimyung Author(s)
Cho, Ho Chan
Department
Dept. of Internal Medicine (내과학)
Journal Title
Obes Facts
Issued Date
2022
Volume
15
Issue
2
Keyword
Extracellular vesicleRNASmall untranslatedObesity
Abstract
Introduction:
Various kidney diseases reportedly show different urinary extracellular vesicle (EV) RNA profiles. Although obesity is one of the main causes of chronic kidney disease, the expression pattern of urinary EV RNA in obesity is uncertain. Our aim was to sequence the small RNA profiles of urinary EVs in obese patients before and after weight reduction and compare them to those of healthy volunteers (HVs).

Methods:
We recruited age-sex-matched obese patients and HVs. The small RNA profiles of urinary EVs were analyzed using RNA sequencing. To evaluate the effect of weight reduction, small RNA profiles of urinary EVs 6 months after bariatric surgery were also analyzed.

Results:
The proportion of urinary EVs transfer RNA and microRNA of obese patients differed from that of HVs. Obese patients showed differential expression of 1,343 small RNAs in urinary EVs compared to HVs (fold change ≥2 and p value <0.05). Among those, 61 small RNAs were upregulated in obese patients and downregulated after weight reduction, whereas 167 small RNAs were downregulated in obese patients and upregulated after weight reduction. RNA sequencing revealed the correlation between the specific urinary EV small RNAs and clinical parameters including body weight, low-density lipoprotein cholesterol, triglyceride, high-density lipoprotein cholesterol, serum glucose, estimated glomerular filtration rate, and albuminuria.

Conclusion:
Obese patients showed distinct urinary EV small RNA profiles compared to HVs. Weight reduction altered urinary EV small-RNA profiles in obese patients.
Keimyung Author(s)(Kor)
조호찬
Publisher
School of Medicine (의과대학)
Citation
Dughyun Choi et al. (2022). Weight Change Alters the Small RNA Profile of Urinary Extracellular Vesicles in Obesity. Obes Facts, 15(2), 292–301. doi: 10.1159/000521730
Type
Article
ISSN
1662-4033
Source
https://www.karger.com/Article/FullText/521730
DOI
10.1159/000521730
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/44210
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
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