계명대학교 의학도서관 Repository

Esophageal Microbiota and Nutritional Intakes in Patients With Achalasia Before and After Peroral Endoscopic Myotomy

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Author(s)
Da Hyun JungYoung Hoon YounDo Hoon KimChul-Hyun LimHee-Sook LimHee Seok MoonJu Yup LeeHyojin ParkSu Jin Hong
Keimyung Author(s)
Lee, Ju Yup
Department
Dept. of Internal Medicine (내과학)
Journal Title
J Neurogastroenterol Motil
Issued Date
2022
Volume
28
Issue
2
Keyword
Esophageal achalasiaMicrobiotaMyotomy
Abstract
Background/Aims:
The composition of the microbiota in the esophagus is only partially understood, especially in patients with achalasia. We aim to investigate the esophageal microbial community and nutritional intakes in patients with achalasia before and after peroral endoscopic myotomies (POEM).

Methods:
Twenty-nine patients were prospectively enrolled from 4 referral institutions across Korea. We collected esophageal samples (mucosal biopsies and retention fluid) and conducted dietary surveys for nutritional intake before and 8 weeks after POEM. The esophageal microbiota was analyzed by 16S rRNA gene sequencing targeting the V3-V4 region.

Results:
Out of the 105 samples from 29 patients, 99 samples were subjected to microbial bioinformatic analysis after quality control, which excluded samples with no amplification or low-quality sequence data. The overall esophageal microbial compositions of patients with achalasia showed that Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria, and Fusobacteria were the dominant phyla, representing over 95% of the total phyla in all groups. At the genus level, Streptococcus was the most abundant in all groups. The observed operational taxonomic unit number was significantly higher in the retention fluid than in the tissue biopsies. However, the esophageal microbial composition showed no significant changes 8 weeks post POEM. The dietary survey analysis showed that nutritional intake significantly improved post POEM.

Conclusion:
This study determined the unique esophageal microbial composition of patients with achalasia, and also found that the microbial composition did not significantly change after POEM in the short-term, despite a significant improvement in the nutritional intake.
Keimyung Author(s)(Kor)
이주엽
Publisher
School of Medicine (의과대학)
Citation
Da Hyun Jung et al. (2022). Esophageal Microbiota and Nutritional Intakes in Patients With Achalasia Before and After Peroral Endoscopic Myotomy. J Neurogastroenterol Motil, 28(2), 237–246. doi: 10.5056/jnm21057
Type
Article
ISSN
2093-0887
Source
https://www.jnmjournal.org/journal/view.html?uid=1755&vmd=Full&
DOI
10.5056/jnm21057
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/44230
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
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