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Thyroid Hormone Ameliorates Lymphedema by Suppressing Adipogenesis in a Murine Lymphedema Model

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Author(s)
Jaehoon ChoiKanghee LeeJunhyung KimWoonhyeok JeongTaehee JoHyoun Wook LeeYoung Sook ParkSang Woo Park
Keimyung Author(s)
Choi, Jae HoonKim, Jun HyungJeong, Woon HyeokJo, Tae Hee
Department
Dept. of Plastic Surgery (성형외과학)
Journal Title
Lymphat Res Biol
Issued Date
2022
Volume
20
Issue
6
Keyword
L-thyroxinelymphedemaadipogenesis3T3-L1 cellmouse
Abstract
Background:
Exogenous supplementation of thyroid hormone could inhibit excessive fat deposition in lymphedema tissue by suppressing adipogenesis.

Methods and Results:
Cell viability, adipogenic differentiation, and mRNA expression were measured in 3T3-L1 preadipocytes treated with L-thyroxine. Twelve mice were divided into control and L-thyroxine groups. Two weeks after lymphedema was surgically induced, the experimental mice were fed L-thyroxine for 4 weeks. Tail volume and body weight were measured, and 6 weeks after the surgery, tail skin and subcutaneous tissue were harvested for histopathologic examination and protein isolation. In 3T3-L1 cells, treatment with 10–500 μM L-thyroxine did not affect cell viability. Eight days after induction of adipogenic differentiation, lipid accumulation decreased significantly in the 50 and 100 μM L-thyroxine groups (p < 0.001). mRNA levels of peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer binding protein α (C/EBPα), and fatty acid-binding protein 4 (FABP4) decreased significantly in the 100 μM L-thyroxine group compared with the control group (p = 0.017). Lymphedema tails treated with L-thyroxine exhibited decreased volume (p = 0.028) and thickness of dermal and subcutaneous tissue (p = 0.01) and increased vascular endothelial growth factor-C protein expression (p = 0.017) compared with the control.

Conclusion:
Thyroid hormone therapy inhibits the adipogenesis of 3T3-L1 cells in vitro and decreases the volume of murine lymphedema tail in vivo. These findings suggest that thyroid hormone therapy could be used to treat lymphedema.
Keimyung Author(s)(Kor)
최재훈
김준형
정운혁
조태희
Publisher
School of Medicine (의과대학)
Type
Article
ISSN
1557-8585
Source
https://www.liebertpub.com/doi/10.1089/lrb.2021.0079
DOI
10.1089/lrb.2021.0079
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/44699
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Plastic Surgery (성형외과학)
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