계명대학교 의학도서관 Repository

Label-free electrochemical detection of glucose and glycated hemoglobin (HbA1c)

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Author(s)
Mukesh ThapaYun Seok Heo
Keimyung Author(s)
Heo, Yun Seok
Department
Dept. of Biomedical Engineering (의용공학과)
Journal Title
Biosens Bioelectron
Issued Date
2023
Volume
221
Keyword
Blood glycemic conditionsDual working screen-printed carbon electrodes (DWSPCEs)Electrochemical detectionGlycated hemoglobin (HbA1c)Gold nano flower (GNF)Label free methods
Abstract
Measuring glucose together with glycated hemoglobin (HbA1c) could provide short-term and long-term information of blood glycemic condition after a single treatment. However, it has been a challenge to quantify glucose and HbA1c from single sample drop and strip of electrodes with suitable sensitivity, selectivity, and efficiency. In this paper, we demonstrated a label free & single sample drop electrochemical detection method of glucose and HbA1c by modifying carbon electrodes. Glucose oxidase and capture antibodies (C-Ab) against HbA1c were immobilized onto the first working electrode (WE1) and the second working electrode (WE2) of dual working screen-printed carbon electrodes (DWSPCEs), respectively. WE2 was modified with Gold Nano Flower. After that, 3-mercaptopropionic acid was coated as a linker. Finally, C-Ab was bonded by the linker. The relationship between gold surface area and concentration of HgAuCl4 was evaluated to optimize HbA1c incubation time. Linear calibration curves for glucose concentration (0.02-35 mM), HbA1c concentration (0.01 to 1mgml-1), and HbA1c percentage solution (0-14%) were obtained, with correlation coefficients of 0.99. Sensitivities of the biosensor for glucose and HbA1c were 0.5 μAmm-2mM-1 and 0.09 μAmm-2μg-1ml, respectively. The biosensor also showed proper stability (>93%, 45days) and selectivity (>92%). The efficiency of the proposed biosensor was also compared with those of commercial kits using whole blood samples of diabetic and normal cases. Results of this study demonstrate that this biosensor can measure both glucose and HbA1c electrochemically using label-free methods to overview glycemic conditions of blood samples using cheap and commercially viable dual carbon electrode-based biosensors.
Keimyung Author(s)(Kor)
허윤석
Publisher
School of Medicine (의과대학)
Type
Article
ISSN
1873-4235
Source
https://www.sciencedirect.com/science/article/abs/pii/S0956566322009472?via%3Dihub
DOI
10.1016/j.bios.2022.114907
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/44742
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Biomedical Engineering (의용공학과)
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