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Effects of Poly-L-Lactic Acid on Adipogenesis and Collagen Gene Expression in Cultured Adipocytes Irradiated with Ultraviolet B Rays

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Author(s)
Hyun-Wook KimYun-A JungJi-Min YunYura KimSung-Ae KimSeong-Il SuhYoung-Wook Ryoo
Keimyung Author(s)
Kim, Sung AeSuh, Seong IlRyoo, Young Wook
Department
Dept. of Dermatology (피부과학)
Dept. of Microbiology (미생물학)
Journal Title
Ann Dermatol
Issued Date
2023
Volume
35
Issue
6
Keyword
AdipogenesisPLLAUVB
Abstract
Background:
Poly-L-lactic acid (PLLA), a synthetic, biocompatible, and biodegradable polymer, has been safely used in several clinical applications. Recently, PLLA has been widely used in the field of dermatology to treat wrinkles in aging skin. Reportedly, PLLA directly acts on dermal fibroblasts causing a significant increase in the expression of type I collagen. However, little is known about the effect of PLLA on adipocytes.

Objective:
This study aimed to analyze the effect of PLLA on adipocytes and examine its potential in treating deep wrinkles engendered by the loss of subcutaneous fat because of aging and photoaging.

Methods:
To elucidate the effect of PLLA on skin photoaging, cultured 3T3-L1 adipocytes were irradiated with ultraviolet B (UVB) rays. Oil red O staining was used to detect lipid accumulation in the adipocytes. Real-time quantitative polymerase chain reaction and Western blotting were performed to detect types IV and VI collagen mRNA and protein levels, respectively, under different conditions.

Results:
The differentiation of 3T3-L1 cells enhanced adipogenesis and the expression of types IV and VI collagens, both of which were inhibited by UVB irradiation. Following this irradiation, PLLA stimulated adipogenesis and the expression of types IV and VI collagens.

Conclusion:
PLLA may provide the beneficial effect on adipocytes from the aspect of adipogenesis and collagen expression in the subcutaneous adipose tissues.
Keimyung Author(s)(Kor)
김성애
서성일
류영욱
Publisher
School of Medicine (의과대학)
Type
Article
ISSN
2005-3894
Source
https://anndermatol.org/DOIx.php?id=10.5021/ad.22.177
DOI
10.5021/ad.22.177
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/45311
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Dermatology (피부과학)
1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
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