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Ribosomal Protein L9 Maintains Stemness of Colorectal Cancer via an ID-1 Dependent Mechanism

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Author(s)
Eun-Hye JeonSo-Young ParkKeon Uk ParkYun-Han Lee
Keimyung Author(s)
Park, Keon UkLee, Yun Han
Department
Dept. of Internal Medicine (내과학)
Dept. of Molecular Medicine (분자의학)
Journal Title
대한암예방학회지
Issued Date
2024
Volume
29
Issue
2
Keyword
Colorectal cancerRibosomal protein L9StemnessInhibitor of DNA-binding 1
Abstract
The identification of therapeutic target genes that are functionally involved in stemness is crucial to effectively cure patients with metastatic carcinoma. We have previously reported that inhibition of ribosomal protein L9 (RPL9) expression suppresses the growth of colorectal cancer (CRC) cells by inactivating the inhibitor of DNA-binding 1 (ID-1) signaling axis, which is functionally associated with cancer cell survival. In addition to cell proliferation, ID-1 is also involved in the maintenance of cancer stemness. Thus, we aimed in this study to investigate whether the function of RPL9 could correlate with CRC stem cell-like properties. Here, we demonstrated that siRNA silencing of RPL9 reduced the invasiveness and migrative capabilities of HT29 and HCT116 parental cell populations and the capacity for sphere formation in the HT29 parental cell population. CD133+ cancer stem cells (CSCs) were then separated from CD133- cancer cells of the HT29 parental cell culture and treated with RPL9-specific siRNAs to verify the effects of RPL9 targeting on stemness. As a result, knockdown of RPL9 significantly suppressed the proliferative potential of CD133+ colorectal CSCs, accompanied by a reduction in CD133, ID-1, and p-IκBα levels. In line with these molecular alterations, targeting RPL9 inhibited the invasion, migration, and sphere-forming capacity of CD133+ HT29 CSCs. Taken together, these findings suggest that RPL9 promotes CRC stemness via ID-1 and that RPL9 could be a potential therapeutic target for both primary CRC treatment and the prevention of metastasis and/or recurrence.
Keimyung Author(s)(Kor)
박건욱
이윤한
Publisher
School of Medicine (의과대학)
Type
Article
ISSN
2288-3657
Source
https://www.jcpjournal.org/journal/view.html?doi=10.15430/JCP.24.004
DOI
10.15430/JCP.24.004
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/45715
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
1. School of Medicine (의과대학) > Dept. of Molecular Medicine (분자의학)
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