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Fulvic acid inhibits the differentiation of 3T3-L1 adipocytes by activating the Ca(2+)/CaMKⅡ/AMPK pathway

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Author(s)
Hyeon Yeong JuSeung-Eun SongSu-Kyung ShinGil-Saeng JeongHo-Chan ChoSeung-Soon ImDae-Kyu Song
Keimyung Author(s)
Cho, Ho ChanSong, Dae Kyu
Department
Dept. of Internal Medicine (내과학)
Dept. of Physiology (생리학)
Journal Title
Biochem Biophys Res Commun
Issued Date
2025
Volume
743
Keyword
3T3-L1 adipocytesFulvic acidCaMKⅡAMPKObesityCytosolic Ca2+
Abstract
Type 2 diabetes increases the risk of developing obesity. Although fulvic acid alleviates back fat thickness in pigs, the mechanism underlying its anti-obesity effect remains unclear. Therefore, we investigated the anti-obesity mechanism of fulvic acid using 3T3-L1 adipocytes. We examined the effects of fulvic acid on adipocyte differentiation, cell viability, and lipid accumulation using molecular techniques. Fulvic acid treatment significantly decreased intracellular lipid accumulation in 3T3-L1 cells during the differentiation compared with that in the control group. Western blotting revealed fulvic acid-induced downregulated expression of the adipocyte differentiation-related markers peroxisome proliferator-activated receptor gamma, CCAAT/enhancer-binding protein alpha, and sterol regulatory element-binding protein 1. The fulvic acid treatment decreased the expression of the lipid uptake-related markers fatty acid-binding protein 4 and the cluster of differentiation 36 in 3T3-L1 cells. Moreover, fulvic acid significantly increased cytosolic Ca2+ concentration via Ca2+ sequestration from the endoplasmic reticulum, enhanced Ca2+/calmodulin-dependent protein kinase II (CaMKII) activity, and upregulated AMP-activated protein kinase (AMPK), thereby reducing adipocyte differentiation. Conclusively, fulvic acid attenuates adipocyte differentiation by activating the Ca2+/CaMKⅡ/AMPK pathway, suggesting its anti-obesity potential.
Keimyung Author(s)(Kor)
조호찬
송대규
Publisher
School of Medicine (의과대학)
Type
Article
ISSN
1090-2104
Source
https://www.sciencedirect.com/science/article/pii/S0006291X24017091
DOI
10.1016/j.bbrc.2024.151173
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/45997
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
1. School of Medicine (의과대학) > Dept. of Physiology (생리학)
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